Sequences from confirmation amplicon


Confirmation sequences for line 774A01 and hit At3g04943 are shown.
Line ID 774A01
Locus At3g04943 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1368459
Primers used for PCR 48qi - 8474, show primer details
   
Sequence Name 49-K115550-0022-774-A01C-48qi
GenBank Accession LN506941 [GenBank]
Sequencing primer gene specific primer, 48qi: 5'-AACCAAAGAATTTGTGATTCCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K115550-0022-774-A01C-48qi
ATCTTATATGTATAAAAAATAGTAAATGATAAAAGAAAAAGAAGGGAAATGATTTAGTTA
ATTATGTCTTGATCATTAATAGATTCAATGGATTAAAGATAACAATAGTTTTGGTCTTTT
GGAGATAGAGATCGAGCTTAACAATCAAACCCACACCAGCATGTAGGATAAAAGAACTCA
GGATGTTGTTTTGAAGGAGGAGATGGGTAATAACATGTTCCATTGCCTTTGTAGTCAGCC
ATGCACTTCTCTTTGCATTTCTCGTCTCCTTCACAAATCCATGAATTACTACACTTTTTT
GCTTCTCCTATCANGATCCCGTTTTCTATATNATAACACAAACACAAGTATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 22-K115549-0022-774-A01C-8409
GenBank Accession LN496249 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K115549-0022-774-A01C-8409
TATACTTGTGTTTGTGTTATTATATAGAAAACGGGATCATGATAGGAGAAGCAAAAAAGT
GTAGTAATTCATGGATTTGTGAAGGAGACGAGAAATGCAAAGAGAAGTGCATGGCTGACT
ACAAAGGCAATGGAACATGTTATTACCCATCTCCTCCTTCAAAACAACATCCTGAGTTCT
TTTATCCTACATGCTGGTGTGGGTTTGATTGTTAAGCTCGATCTCTATCTCCAAAAGACC
AAAACTATTGTTATCTTTAATCCATTGAATCTATTAATGATCAAGACATAATTAACTAAA
TCATTTCCCTTCTTTTTCTTTTATCATTTACTATTTTTTATACATATAAGATTTTATTAT
AGGAGTTAAGACGATGAAGAAGGAATATTAGAGATGGAATCACAAATTCTTTGGT

Last Updated on 06.12.2018 16:11