Sequences from confirmation amplicon


Confirmation sequences for line 778D03 and hit At5g39500 are shown.
Line ID 778D03
Locus At5g39500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15817894
Primers used for PCR 84zl - 8474, show primer details
   
Sequence Name 15-K130850-0022-778-D03C-84zl
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 84zl: 5'-ATAGCAGAGATATAATCGTTCGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K130850-0022-778-D03C-84zl
TTTTCAGACGACAACTTCTTGTGCAATGCGTTCACAGTATGCATCAGCCACTTTTGCTTT
CAGCTTGAGAACCAGTTGGAGTGATTTCAGAAGTTCATCTGTTAGGTTTTCCTTGTATGG
TAATAATCTCTGGCAAATCTTCAGTACACCAAAAACAGCCTTTTCAACAAGCGTGCATGG
TGTTAATGTTAATTGAACTATACCCAAGATATGCTCATAAACTGTTGGCCAAATCAGTAG
GATTCTGTCTCGGTTGTTCAGCGTTACCGCAATCAGAAGCTCCAGGCAAAAGACCGAACT
AGCTTCATCTTTCCCGGATGCTCTTATAAGAGAGTTAACAAGCTGCTGCAGAGACTCAGC
TTGGAGAAACTTGCTGTCTGAGAATATGCTATCAATATGACAATCTTTCACTATCCCACG
AGCGTGTTTATAAGCTGCCAATTCTTCCTCGCTTGGTAATGGCTTTGTTTCCTCAGAATC
AAAAGACAGAAGAAACCGACCAATAAAACTAGATGATTTTCGAGGCATCGCTGAGGGTTG
TGACTGTGATACGACTGGCACGGGATTTGCCGAAGGTTTTTCTTGCTCCAAGTTACTAGT
TGACAGCTCTGGGTCATCCGCTGCATCACTAGCTATATGATCTGGTAAAATGTGGAGTTT
GTTGAGGCTCAAGACACATTCCAGTATGTTTTTCCAGCCCGCACTAATATAGTCACCATA
TTTATTTGCAATCAAGAAAACAGCTTCAGTAGCCATTCTGGCTCTGGCATCTTCTCCTAG
AACAAGAACAGCTTCATCAGCAGATAATGGGGCGAAGAAGGGTGTGAATTTGCAGAGAGA
CACGACCAGATCATCCAAACACTATTCAAATGATAATATGCTG
   
Sequence Name 06-K130849-0022-778-D03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K130849-0022-778-D03C-8409
CATTTACATTGAACGAGTCCCTATATCCAATGTGACGCAGCATCCCATCTGGACCGTGAC
ATGTTTTATATCGTGTCTGGTCCCACTATTGCTGCTACATCAGTGGTTTTTGAGCAAGCA
GAGCAAGAAGATGTTTTGCGGAGATGTATTGATGGGTTATTGGCCATTGCAAAACTTTCA
GCATATTATCATTTGAATAGTGTTTTGGATGATCTGGTCGTGTCTCTCTGCAAATTCACA
CCCTTCTTCGCCCCATTATCTGCTGATGAAGCTGTTCTTGTTCTAGGAGAAGATGCCAGA
GCCAGAATGGCTACTGAAGCTGTTTTCTTGATTGCAAATAAATATGGTGACTATATTAGT
GCGGGCTGGAAAAACATACTGGAATGTGTCTTGAGCCTCAACAAACTCCACATTTTACCA
GATCATATAGCTAGTGATGCAGCGGATGACCCAGAGCTGTCAACTAGTAACTTGGAGCAA
GAAAAACCTTCGGCAAATCCCGTGCCAGTCGTATCACAGTCACAACCCTCAGCGATGCCT
CGAAAATCATCTAGTTTTATTGGTCGGTTTCTTCTGTCTTTTGATTCTGAGGAAACAAAG
CCATTACCAAGCGAGGAAGAATTGGCAGCTTATAAACACGCTCGTGGGATAGTGAAAGAT
TGTCATATTGATAGCATATTCTCAGACAGCAAGTTTCTCCAAGCTGAGTCTCTGCAGCAG
CTTGTTAACTCTCTTATAAGAGCATCCGGGAAAGATGAAGCTAGTTCGGTCTTTTGCCTG
GAGCTTCTGATTGCGGTAACGCTGAACAACCGAGACAGAATCCTACTGATTTGGCCAACA
GTTTATGAGCATATC

Last Updated on 06.12.2018 16:11