Sequences from confirmation amplicon


Confirmation sequences for line 779D08 and hit At1g61030 are shown.
Line ID 779D08
Locus At1g61030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22481542
Primers used for PCR 75ra - 8474, show primer details
   
Sequence Name 50-K115914-0022-779-D08C-75ra
GenBank Accession LN507282 [GenBank]
Sequencing primer gene specific primer, 75ra: 5'-ACGGAATAAACAGGTACCAAACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K115914-0022-779-D08C-75ra
AACATAGTCACTGATTGTCTTTTTGCAAATGGGGGGAACTCGTGATTTCATAATATTATG
ATCTGACTCGTACCCATGTCTTGAAAAATATTCGCTAATCAGTTATTATTTGGTCCAGGT
CAAGGCTCGCGGCAGCTAGTGTTCCGATTACCAATCCAGAAGGGTTACAAGATAGTGAAC
AAGACATGATCCCTCTTTTATGTTCAATCTTTCTCACCAATAAAGGATCCGCAGACACCA
AAGATGAAACGAGTACTTTCACTTTACAATTGAATATATCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 24-K115913-0022-779-D08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K115913-0022-779-D08C-8409
TGGATAAGAAGAAAGAGTAATTACCAATTTTTTTTCACTTCAAAAATGTAGATGT

Last Updated on 06.12.2018 16:11