Sequences from confirmation amplicon


Confirmation sequences for line 779F09 and hit At1g64760 are shown.
Line ID 779F09
Locus At1g64760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24055823
Primers used for PCR 43cs - 8474, show primer details
   
Sequence Name 10-K108747-0022-779-F09C-8409
GenBank Accession LN490694 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K108747-0022-779-F09C-8409
CGGACTGAGCCATTTACATTGAATATTGAGACAGAACAGAGAGGAAGAGAGACAAACTCA
CGTGATATTGACACCACCATCGAAGTTATAACGAGTGACATTCTTACGAACCCAATCTTT
GGCTCGGTCGTAACTAGTCATAACCTTAAGCTGATCATTGGGAATAGCGACCATCACTTC
AAGACCCGATCCAGCGAGTGCACCCATGGTTGTCTCGTCCGCATCAAAAAGCTTAACTTT
GTTGATGTTATTGTCTTTAAGCATCTGAACCACTGTCTTTGGAGGTAGCTTATGTGTAGC
CATTGTTCCCCAATTCACACCCAACCCATTTACCAAAATACCCAAATGACCAA
   
Sequence Name 13-K108748-0022-779-F09C-43cs
GenBank Accession LN492075 [GenBank]
Sequencing primer gene specific primer, 43cs: 5'-ATTGGTCATTTGGGTATTTTGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K108748-0022-779-F09C-43cs
GCTACCTCCAAGACAGTGGTTCAGATGCTTAAAGACAATAACATCAACAAAGTTAAGCTT
TTTGATGCGGACGAGACAACCATGGGTGCACTCGCTGGATCGGGTCTTGAAGTGATGGTC
GCTATTCCCAATGATCAGCTTAAGGTTATGACTAGTTACGACCGAGCCAAAGATTGGGTT
CGTAAGAATGTCACTCGTTATAACTTCGATGGTGGTGTCAATATCACGTGAGTTTGTCTC
TCTTCCTCTCTGTTCTGTCTCAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT

Last Updated on 06.12.2018 16:11