Sequences from confirmation amplicon


Confirmation sequences for line 780C03 and hit At5g06050 are shown.
Line ID 780C03
Locus At5g06050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1820852
Primers used for PCR 06ek - 8474, show primer details
   
Sequence Name 27-K110713-0022-780-C03C-06ek
GenBank Accession LN498440 [GenBank]
Sequencing primer gene specific primer, 06ek: 5'-GGTAATGCTTCTGAGAATTGGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K110713-0022-780-C03C-06ek
ATCTGCTCGAACTCTATTTTCTTCAGATCTCCGCCTTTGTTTTGATCTCCGTTGCTTGTT
TCTTCCTCGGTAAGCATTGGTCTGAGGATGGCTTCCGCCGTCTCATCTTCTTCTCCGCCG
AGCCTTCCCGATCTCCCATTGTCGCACTCTCACCTGATTTCGGAAAAACCTATAACATCT
CTGGTTTGATCTATGAGAGTCACCCGATTTTGCCTCCGTCTTTGTCACCACCGCCGCCGC
CAGATTCTGTTGAGTTGAAGGTGTTTGGAATCGTTAATGAGAACGGAACAATGTCTGATG
AGTTTCAGATTGGTGATTACGACGTTGAGTCGGCGGAGACGTTGGGGAATCAGACGGAAT
TCGAGAGTAGCGACGACGATGATATTAAATCCACCACAGCTAGAGTTAGTGTGAGGAAAT
TTGAGATTTGTTCGGAGAATATGACTGAGTACATTCCTTGTTTGGATAATGTAGAAGCCA
TCAAAAGGCTTAATTCAACGGCGCGTGGTGAGCGATTCGAACGGAATTGTCCTAACGATG
GAATGGGATTGAATTGCACCGTTCCGATTCCTCAAGGATACCGTAGTCCGATTCCGTGGC
CGAGAAGCCGTGATGAGGTATATCTGTGACGCAATGATATATTCAATTGTAATCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGC
   
Sequence Name 11-K110711-0022-780-C03C-8409
GenBank Accession LN491168 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K110711-0022-780-C03C-8409
CGGAATGAGCCATTTACATTGAATATATCCTGATTACAATTGAATATATCATTGCGTCAC
AGATATACCTCATCACGGCTTCTCGGCCACGGAATCGGACTACGGTATCCTTGAGGAATC
GGAACGGTGCAATTCAATCCCATTCCATCGTTAGGACAATTCCGTTCGAATCGCTCACCA
CGCGCCGTTGAATTAAGCCTTTTGATGGCTTCTACATTATCCAAACAAGGAATGTACTCA
GTCATATTCTCCGAACAAATCTCAAATTTCCTCACACTAACTCTAGCTGTGGTGGATTTA
ATATCATCGTCGTCGCTACTCTCGAATTCCGTCTGATTCCCCAACGTCTCCGCCGACTCA
ACGTCGTAATCACCAATCTGAAACTCATCAGACATTGTTCCGTTCTCATTAACGATTCCA
AACACCTTCAACTCAACAGAATCTGGCGGCGGCGGTGGTGACAAAGACGGAGGCAAAATC
GGGTGACTCTCATAGATCAAACCAGAGATGTTATAGGTTTTTCCGAAATCAGGTGAGAGT
GCGACAATGGGAGATCGGGAAGGCTCGGCGGAGAAGAAGATGAGACGGCGGAAGCCATCC
TCAGACCAATGCTTACCGAGGAAGAAACAAGCAACGGAGATCAAAACAAAGGCGGAGATC
TTGAAGAAAATAGAGTTTCTGAGCAGATTGCTATTGAGGAAGAGCTTCATTAGCTTCCAA
TTCTCAAAGCCATTA

Last Updated on 06.12.2018 16:11