Sequences from confirmation amplicon


Confirmation sequences for line 781E10 and hit At5g49560 are shown.
Line ID 781E10
Locus At5g49560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20111196
Primers used for PCR 19hg - 8474, show primer details
   
Sequence Name 33-K112479-0022-781-E10C-19hg
GenBank Accession LN501056 [GenBank]
Sequencing primer gene specific primer, 19hg: 5'-TCAAAGTCTGTGTGACTGTGTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K112479-0022-781-E10C-19hg
TTTTTAGTATTTCAAAATAGTCCTAGTAATTGTACGATTCACAAAAATAGGCCCCAAGCT
ATTTATGGTCGCAACCCNCTTTAAAAACTTATTTGCTTTATCACCTATAGTTTTTGATCA
TTTATAAAAGAGTCCAAATAGTTTGTAAGGCCTTATAGTCTTCAATGCTTATATTTAATT
TGCATGGATTGGTTCATCATTTAATAACACCTGAATTGAATAGACATGACAAACATTTTT
GCAAACTTTATTTAGGAAATGAATCCTGACAACGCGATTGCTAACACGAAACAATCCGAC
CATTCTGATTTGGCTGTTTCGTGGTGAAACGGTAAACCACAACACCGATTCTAGAACTTT
CTTGAGGAACATCAGAGTGGATAACGTCAACATCGAAGAGCTTTCGAGCTTTTTTGAAGA
AGACAGATTCTTTCTTCCATCTCCTTAGATGCGCCATCAGGAAAATTAACCTCTTCCCCT
CTNGCTGCATCAAACGCAGCGTTTTGAGGAGAGGCTGCATCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTT
A
   
Sequence Name 03-K112478-0022-781-E10C-8409
GenBank Accession LN487553 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K112478-0022-781-E10C-8409
GCATTTCATTGAATATATCCTGATGCAGCCTCTCCTCAAAACGCTGCGTTTGATGCAGCT
AGAGGGGAAGAGGTTAATTTTCCTGATGGCGCATCTAAGGAGATGGAAGAAAGAATCTGT
CTTCTTCAAAAAAGCTCGAAAGCTCTTCGATGTTGACGTTATCCACTCTGATGTTCCTCA
AGAAAGTTCTAGAATCGGTGTTGTGGTTTACCGTTTCACCACGAAACAGCCAAATCAGAA
TGGTCGGATTGTTTCGTGTTAGCAATCGCGTTGTCAGGATTCATTTCCTAAATAAAGTTT
GCAAAAATGTTTGTCATGTCTATTCAATTCAGGTGTTATTAAATGATGAACCAATCCATG
CAAATTAAATATAAGCATTGAAGACTATAAGGCCTTACAAACTATTTGGACTCTTTTATA
AATGATCAAAAACTATAGGTGATAAAGCAAATAAGTTTTTAAAAGGGGTTGCGACCATAA
ATAGCTTGGGGCCTATTTTTGTGAATCGTACAATTACTAGGACTATTTTGTAAATACTTA
AAAACTATTGGAACAAAAGTATCAGAGTAGAACTTGTGACACACAGTCACACGNACTTTG
A

Last Updated on 06.12.2018 16:11