Sequences from confirmation amplicon


Confirmation sequences for line 782B07 and hit At3g21180 are shown.
Line ID 782B07
Locus At3g21180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7427079
Primers used for PCR BE63 - 8474, show primer details
   
Sequence Name 57-K028750-022-782-B07C-8409
GenBank Accession LN509307 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K028750-022-782-B07C-8409
TATACCTCTTAGGATAGGTGACCAGGTAGGTATACTTTGTCTTTATATTCCATCTAAGGG
CTTCAGAGTTAAATGACTATATGGCCTTATTTATTGTTGTATTCATATAGGTCCCTGCGG
ATGGAGTGCTAATTAGTGGTCATTCTCTTGCTATTGATGAATCTAGCATGACCGGTGAAA
GCAAGATTGTGAGTTCATTATGGTTCTGCTTGTGCAATTTTTATGGTCATCTTCCCTTTC
TATTTGCTAACACAGACCTTTGTTTGCAGGTTCACAAGGATCA
   
Sequence Name 57-K028750-022-782-B07C-BE63
GenBank Accession LN509308 [GenBank]
Sequencing primer gene specific primer, BE63: 5'-TTAGAAAAGGGGATTTTTGATCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K028750-022-782-B07C-BE63
GCAATAGAAAGGGAAGATGACCATAAAAATTGCACAAGCAGAACCATAATGAACTCACAA
TCTTGCTTTCACCGGTCATGCTAGATTCATCAATAGCAAGAGAATGACCACTAATTAGCA
CTCCATCCGCAGGGACCTATATGAATACAACAATAAATAAGGCCATATAGTCATTTAACT
CTGAAGCCATTAGATGGAATATAAAGACAAAGTATACCTACCTGGTCACCTATCCTAAGA
GGTATAACATCTCCGACAACAACGTCATAGATTGATATCTTCACTGTTCTCCCTCCTCTC
ATGACCTGTAAGAGTTTTCGTTTATTTAATCTTCAGCTCAAAAACATGTAATTGCAAAAC
AAACTAAGATGTTAAAGCAAGCACCTCTAGTTGTATATTTCTTTTTTCATCGTTGAGGTT
TTGAAACTGGAGAAAAAGTGGCTTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11