Sequences from confirmation amplicon


Confirmation sequences for line 784G06 and hit At5g25780 are shown.
Line ID 784G06
Locus At5g25780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8974499
Primers used for PCR 60xc - 8474, show primer details
   
Sequence Name 40-K119373-0022-784-G06C-60xc
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 60xc: 5'-GTTTTTGAATAAGTTCCACTGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K119373-0022-784-G06C-60xc
TTTCAGTGATTATGTGTTTACCAGCTGAATTAGTGTTAGGATCTGTTTACGTATTTGATG
TATAGTAACCAATAAATAATAATTTCATCAGTCTTGTTAAATGTTTATTCTTTTTTATTC
AATGCAGCCTTTTGAGGTGCTTGGTCTTGATACTTATATGGTGTTTGTTTTTCAGGAAAA
TTTGCAGAAGTGGCTTACTGATGACAAAGCCCGGGACCAGCTAGTCATTCGTTTTGGCCA
TGATACTGAAGTTTACTGGAATGATGCTAGGCAGAAGAAGCCTGAACCTGTTCATAAGCG
TTCTGTAAGTTACATCTGTGTCAGTTTAGAGCAATGTTATATGTTTATGTTACCATCATC
TGAATTAATGATGTTGTCTTCCTATGCAGTATTGGACAGAGAGTTATGTGCAGTGGTCTC
CAATAGGTACATACCTTGTGACACTTCACAAGCAGGGGGCAGCTGTTTGGGGTGGTGCTG
ATACATTTACGCGTCTCATGCGTTATCAGCATTCAATGGTTTGAAATTCCTTTCAATTGC
TACATAGTACTTCCGTTTTTCCTGTGTTGGTCTGAAAATTGACTTCTTTTGTTGATTCCG
TTTTAGGTCAAACTAGTTGATTTCTCGCCTGGTGAGAAATATCTTGTAACTTACCATAGC
CAAGAACCAAGCAATCCGAGAGATGCAAGTGTGAGTTCATCTTTGCCTCTGTTCTTTCCA
ACTTTTATTCGTATAACATTTCTATGCAAAGAATTCTGATATTTGTCAAAATTTCAGAAA
GTAGAGATTAANGTCTTTGATGTGAGAACCGGGAGGATGATGAGAGATTTCAGGGTAGTG
CTGATGA
   
Sequence Name 08-K119372-0022-784-G06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K119372-0022-784-G06C-8409
ATCTGCCAGTACATCTTGCAGTCGCTCACACTGAAGAGATTCTTCTGCCTCAGCTCTACC
TTACTCGGGATTTGGACTAGAGCAACCTGAAGAATGGGATATCAACATGTAAATACAAAA
CAACTGGCAGAGAAACGGAAGATTATTGAGGTATCAGGGCTTTTCATTTACATAAGAAAT
AAAAGAAACATACCTTGGCTGGTTGGTTTCCACCACCTTGTTCAGGAACAAACAATGAAA
GGATTGAATCAGTGGGAGACCAGCAAATATCCACAACATTATCAACCTTCATGGATTTCT
TGTCTATTAGGCTGAAAGTCTCAGTCTCATAGACAGAGATAGTGTTTTTGCTCAGCTTGG
CAAAGTATTTATCATCTTTTCCGCCAGCCCATCTGAAAAAAGTTGGCAAGATATCAACAA
ATTTGCATAATAGTGGAATAGAGGAGAGAAATTGACTATTATTTTTAGTACATTGACATG
CGGTTGAAGAAATTACCTGAAAACAGGCCAAGAGGCACCAGCAACACCACCAGGTCCTCC
AATTGAAAACTCATCAGCACTACCCTTGAAATCTCTCATCATCCTCCCGGTTCTCACATC
AAAGACCTTAATCTCTACTTTCTGAAATTTTGACAAATATCAGAATTCTTTGCATAGAAA
TGTTATACGAATAAAAGTTGGAAAGAACAGAGGCAAAGATGAACTCACACTTGCATCTCT
CGGATTGCTTGGTTCTTGGCTATGGTAAGTTACAAGATATTTCTCACCAGGCGAGAAATC
AACTAGTTTGACCTAAAACGGAATCAACAAAAGAAGTCAATTTTCAGAACCACACAGGAA
AAACGGAAGTACTATGTAGCAATTGAAAGGAATTCAAAC

Last Updated on 06.12.2018 16:11