Sequences from confirmation amplicon


Confirmation sequences for line 785A01 and hit At1g78100 are shown.
Line ID 785A01
Locus At1g78100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29387932
Primers used for PCR MN41 - 8474, show primer details
   
Sequence Name 14-K117142-0022-785-A01C-MN41
GenBank Accession LN492685 [GenBank]
Sequencing primer gene specific primer, MN41: 5'-TTAGCTGGTTTGGAGAAGATAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117142-0022-785-A01C-MN41
TCGGAGAGCTAGCGATAGGAGAATCGATCTCGGAGTCAGATTCGGTGGCTCCGAGGATCT
GATCGAGTTGGAGAAGGAGCGACTCAGACTGAGTGGCTAACGAGTTGAATCGTTTAGAAA
CGGAACGACATCGTATTAACGTTTTGACGTCACCGACTCTGTTCAAAATGTCGATGACCA
CAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATAAT
   
Sequence Name 04-K117141-0022-785-A01C-8409
GenBank Accession LN488084 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K117141-0022-785-A01C-8409
GCATTTCATTGGATATATCCTGTGGTCATCGACATTTTGAACAGAGTCGGTGACGTCAAA
ACGTTAATACGATGTCGTTCCGTTTCTAAACGATTCAACTCGTTAGCCACTCAGTCTGAG
TCGCTCCTTCTCCAACTCGATCAGATCCTCGGAGCCACCGAATCTGACTCCGAGATCGAT
TCTCCTATCGCTAGCTTCTTCCGATCTCTCTTCAAATCCATTCACGGTCTCCTTCCTCCT
ATCTTCTCCANACCAGCTAA

Last Updated on 06.12.2018 16:11