Sequences from confirmation amplicon


Confirmation sequences for line 785A08 and hit At2g30310 are shown.
Line ID 785A08
Locus At2g30310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12923865
Primers used for PCR NE87 - 8474, show primer details
   
Sequence Name 05-K102498-0022-785-A08C-8409
GenBank Accession LN488304 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K102498-0022-785-A08C-8409
TTACAATTGAATATATCCTGGTATATCAGTGATTGAATATACTCATTGCTGATCCATATA
GTTTAGGTTACCACCAATGGGATGTTTACCAATCCAAATGACCGCGAAAATGAGAAACAT
TTTGAGATTTTGTGTGGAACAAGAAAACAAAGACTCCGTTTTGTACAATCAGAAACTAGT
AAAGAAACTGCCTGAGATCCAAGCGTCTCTACCGGGAAGCAATTTCCTTTACGCCAACGT
CTACGACCCATTGATGGACATGATCCAAAACCCTAGTAAATACGGTATATACCATTTCTT
CCTTGTAACGCAAGTTATCTAAATCTCAATCTTAATAATTTATGCAATAGATACTCATTG
TTGTACACATGAAAACAGGATTCAAGGAGACTAAGAAAGGATGTTGCGGAACAGGATACT
TGGAAACAACATTCATGTGCAATCCCCTTACAAAGACTTGTCCGAATCACTCCGATCACT
TGTTTTGGGACTCAATTCATCCCTCGGAAGCTGCCTACAATTACTTGGGCNACTTTG
   
Sequence Name 05-K102499-0022-785-A08C-NE87
GenBank Accession LN488305 [GenBank]
Sequencing primer gene specific primer, NE87: 5'-CAAAGTTGCCAATGTAATTGTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K102499-0022-785-A08C-NE87
TTCGGACAAGTCTTTGTAAGGGGATTGCACATGAATGTTGTTTCCAAGTATCCTGTTCCG
CAACATCCTTTCTTAGTCTCCTTGAATCCTGTTTTCATGTGTACAACAATGAGTATCTAT
TGCATAAATTATTAAGATTGAGATTTAGATAACTTGCGTTACAAGGAAGAAATGGTATAT
ACCGTATTTACTAGGGTTTTGGATCATGTCCATCAATGGGTCGTAGACGTTGGCGTAAAG
GAAATTGCTTCCCGGTAGAGACGCTTGGATCTCAGGCAGTTTCTTTACTAGTTTCTGATT
GTACAAAACGGAGTCTTTGTTTTCTTGTTCCACACAAAATCTCAAAATGTTTCTCATTTT
CGCGGTCATTTGGATTGGTAAACATCCCATTGGTGGTAACCTAAACTATATGGATCAGCA
ATGAGTATATTCAATCACTGATATACCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCCTC

Last Updated on 06.12.2018 16:11