Sequences from confirmation amplicon


Confirmation sequences for line 785F02 and hit At2g20100 are shown.
Line ID 785F02
Locus At2g20100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8680998
Primers used for PCR 25wm - 8474, show primer details
   
Sequence Name 49-K118874-0022-785-F02C-25wm
GenBank Accession LN507001 [GenBank]
Sequencing primer gene specific primer, 25wm: 5'-TGTGATCTAGTGGGAGTATTGGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K118874-0022-785-F02C-25wm
CCCAATTCTTTTGCGGTGGGTCGGCCTGTATTACATGTAAATGATATATGGATAAAATAA
CCTATGCCAGTTTCATGAATATTTACTGTTTTGCAACCAAAAAATGAATATTTACTGTTT
TGTGCACCAATCCGCGGGTTTAATACCTTTGAGTGTGAGTATTTTGTATTTAATTTTCTG
AATTTGAAAAGCGATAAATTTTGCTTTGAAAAATATTGCTAAAATTTTGAGTCAGAGTTA
ATTTCAAACTCACAAGTAGTCACAAAATATAAAACCATTTGTGGGACAGGTGAATCGTGA
TATCTTGGTTGGAAAAGTTGATTTAGATCGATCATTTGCCAAAAAAACTTTTGTGACCAA
AGCTACTCTGATTGAGGAAAATAAATATAATAAATCATTTAAATGGCTTTTCTAGGTTTA
ATAGAGGACTGAAAAGGAAAGTTGGTAATGTCATCTTTTTATTTTATTGACCATACTTTA
TCGTAGTCATTTCAATTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCC
   
Sequence Name 09-K118873-0022-785-F02C-8409
GenBank Accession LN490415 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K118873-0022-785-F02C-8409
CCATTTACATTGAAATTGAAATGACTACGATAAAGTATGGTCAATAAAATAAAAAGATGA
CATTACCAACTTTCCTTTTCAGTCCTCTATTAAACCTAGAAAAGCCATTTAAATGATTTA
TTATATTTATTTTCCTCAATCAGAGTAGCTTTGGTCACAAAAGTTTTTTTGGCAAATGAT
CGATCTAAATCAACTTTTCCAACCAAGATATCACGATTCACCTGTCCCACAAATGGTTTT
ATATTTTGTGACTACTTGTGAGTTTGAAATTAACTCTGACTCAAAATTTTAGCAATATTT
TTCAAAGCAAAATTTATCGCTTTTCAAATTCAGAAAATTAAATACAAAATACTCACACTC
AAAGGTATTAAACCCGCGGATTGGTGCACAAAACAGTAAATATTCATTTTTTGGTTGCAA
AACAGTAAATATTCATGAAACTGGCATAGGTTATTTTATCCATATATCATTTACATGTAA
TACAGGCCGACCCACCGCAAAAGAATTTGGGCTGTTGCGAAGTAGGCCAACCCGTTTTGT
CACCAATACTCCA

Last Updated on 06.12.2018 16:11