Sequences from confirmation amplicon


Confirmation sequences for line 786C06 and hit At1g16170 are shown.
Line ID 786C06
Locus At1g16170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5540006
Primers used for PCR 14vs - 8474, show primer details
   
Sequence Name 51-K118848-0022-786-C06C-14vs
GenBank Accession LN507651 [GenBank]
Sequencing primer gene specific primer, 14vs: 5'-AACAGATCCGAAGTAGGTGAAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K118848-0022-786-C06C-14vs
GTTAGAGATCTGGAGAGAAAAAGCTAGATTCCAGCTCCGTCGACAACCCCCGAAATCTTC
AGCAGTGTTGTTGCGATATGGAGAGAGAGAGAGAGATGGATCTCCTCCGTAGATTGATGA
TGGCTCGAGCTGGGCCGATTTGCCGGATACGCCGTACGCGGCTTATTATGTCCGTTACAT
AAAACGGGAATTTAGGGATTTTATATACAAAACAATAAAAAGATAAAAAATAGGAAAGAA
GATATATAAAGAGGACAAAAAAATGATGATGCGTTGTTTGTTAGCTTCCACTCCTTTGTG
TTTTGAAGATGATGGGCATGTTGTTAGAATCCGATGATGAGTGATGACATTTACCTAGGA
CTATGATCTGACTCTTGCAGCTTCCAGAGGGCATTTACGAATACGTATTAAGATAAACTT
TTTTAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTC
   
Sequence Name 38-K118847-0022-786-C06C-8409
GenBank Accession LN503164 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K118847-0022-786-C06C-8409
TTAAAAAAGTTTATCTTAATACGTATTCGTAAATGCCCTCTGGAAGCTGCAAGAGTCAGA
TCATAGTCCTAGGTAAATGTCATCACTCATCATCGGATTCTAACAACATGCCCATCATCT
TCAAAACACAAAGGAGTGGAAGCTAACAAACAACGCATCATCATTTTTTTGTCCTCTTTA
TATATCTTCTTTCCTATTTTTTATCTTTTTATTGTTTTGTATATAAAATCCCTAAATTCC
CGTTTTATGTAACGGACATAATAAGCCGCGTACGGCGTATCCGGCAAATCGGCCCAGCTC
GAGCCATCATCAATCTACGGAGGAGATCCATCTCTCTCTCTCTCTCCATATCGCAACAAC
ACTGCTGAAGATTTCGGGGGTTGTCGACGGAGCTGGAATCTAGCTTTTTCTTCTCCAGAT
CTCTAAACCGATTCTCACCGGTTCGTTTTCTAATAATCTTTCACCTACTC

Last Updated on 06.12.2018 16:11