Sequences from confirmation amplicon


Confirmation sequences for line 787E04 and hit At3g45810 are shown.
Line ID 787E04
Locus At3g45810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16837497
Primers used for PCR MJ44 - 8474, show primer details
   
Sequence Name 51-K029917-022-787-E04C-8409
GenBank Accession LN507391 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K029917-022-787-E04C-8409
AAAGGCGATCGTTCTGTGAAACAACCCGAAAGCACAATCAATAGCAATAATCCTGAGAGT
AGTGGTGCTGGTGGTGGGATCTTGAAGAAATGTGTCTAAGAATCTCGCCGTGGGATCAAT
AATCAGGTCCATGAGCGTAAACAAATGGAGAAAGAGCGGTAATCTCGGGTCCCCGAGCAC
GAGGAAGAGTGGAAACCTGGGGCCTCCACTCCCTGTGTCCCAAGTAAAAAGACCGGGGCC
ACAAAGAGTCGAGAGGACTACATCTAGCGCGGCTAGAGGACTACAAAGTCTCCGGTTCCT
TGATCGGACCGTCACGGGAAGAGAACGAGATTCGTGGAGATCAATCGAAAACCGGTTCAA
TCAATTTGCTGTTGACGGGAGGCTTCCCAAGGATAAATTCGGCGTTTGCATTGGTAAAAC
TATAATTCATCCATTTTTTTACGTAAACCTAATTTAAAAAACTACGGTTATACATATGTT
TTTTTGTCAACTTTAAAAGGTTGTTCTTGATCAAACTGAAACAAAAAGATGTTTTCATAT
TATAATTATGTGTGTATTAGGAAT
   
Sequence Name 51-K029917-022-787-E04C-MJ44
GenBank Accession LN507392 [GenBank]
Sequencing primer gene specific primer, MJ44: 5'-CTCCAATGTATCTCCCATTCCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K029917-022-787-E04C-MJ44
AAATGAAACATCTTTTTGTTCAGTTTGATCAAGAACAACCTTTTAAAAGTTGACAAAAAA
ACATATGTATAACCGTAGTTTTTTAAATTAGGTTTACGTAAAAAAATGGATGAATTATAG
TTTTACCAATGCAAACGCCGAATTTATCCTTGGGAAGCCTCCCGTCAACAGCAAATTGAT
TGAACCGGTTTTCGATTGATCTCCACGAATCTCGTTCTCTTCCCGTGACGGTCCGATCAA
GGAACCGGAGACTTTGTAGTCCTCTAGCCGCGCTAGATGTAGTCCTCTCGACTCTTTGTG
GCCCCGGTCTTTTTACTTGGGACACAGGGAGTGGAGGCCCCAGGTTTCCACTCTTCCTCG
TGCTCGGGGACCCGAGATTACCGCTCTTTCTCCATTTGTTTACGCTCATGGACCTGATTA
TTGATCCCACGGCGAGATTCTTAGACACATTCTTCAAGATCCCACCACCAGCACCACTAC
TCTCAGGATTATTGCTATTGATTGTGCTTTCGGGTTGTTTCACAGAACTATCGCCTTTTG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTA

Last Updated on 06.12.2018 16:11