Sequences from confirmation amplicon


Confirmation sequences for line 787E10 and hit At3g01500 are shown.
Line ID 787E10
Locus At3g01500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:196605
Primers used for PCR BU61 - 8474, show primer details
   
Sequence Name 11-K104352-0022-787-E10C-BU61
GenBank Accession LN491095 [GenBank]
Sequencing primer gene specific primer, BU61: 5'-CTGAACAAGCAAACACCATGTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K104352-0022-787-E10C-BU61
TTTTTCTATGTTTCATTTTTAATTTATAAGCAACCATGATATACACATAGTATTAGTTTT
ATTTTTAATTTACCTTAGGACTTTGACCCTTTGCGAGCTCACCGTACAAAGCAGGGTTGG
TTCTGCAAATAAAAGTATAAATATGAACCAGTTAATTAATCTAGACCACTTTAGTTGGTG
ACATAATTAAGAACAAACAAATGAAATAGAAATATATATTACTCGTATTTCTCCTTCTTG
AATTTGATGAAGCCCTGCTTAATGGTTTCGACGGGGTCGAAAGCTTTCTTGTCGGATGAA
GTACCTGTCTGAAGAGCCGCTGTGATCTGCTCCACCTTTGCCGCTGCAACCGTCTTTAGC
TCTTCCTTCTCGCTGCCATATGTTTATATACATTTATAGTTAAATCACAAAAATTATGAA
TTAAGTAGTATTGAAAAATCCCGTTACTAATTTATAATAATGTTTTAGCTTTATTCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAC
   
Sequence Name 27-K104353-0022-787-E10C-8409
GenBank Accession LN498366 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K104353-0022-787-E10C-8409
CATTTACAATTGAATATATCCTGAATAAAGCTAAAACATTATTATAAATTAGTAACGGGA
TTTTTCAATACTACTTAATTCATAATTTTTGTGATTTAACTATAAATGTATATAAACATA
TGGCAGCGAGAAGGAAGAGCTAAAGACGGTTGCAGCGGCAAAGGTGGAGCAGATCACAGC
GGCTCTTCAGACAGGTACTTCATCCGACAAGAAAGCTTTCGACCCCGTCGAAACCATTAA
GCAGGGCTTCATCAAATTCAAGAAGGAGAAATACGAGTAATATATATTTCTATTTCATTT
GTTTGTTCTTAATTATGTCACCAACTAAAGTGGTCTAGATTAATTAACTGGTTCATATTT
ATACTTTTATTTGCAGAACCAACCCTGCTTTGTACGGTGAGCTCGCAAAGGGTCAAAGTC
CTAAGGTAAATTAAAAATAAAACTAATACTATGTGTATATCATGGTTGCTTATAAATTAA
AAATGAAACATTAAGAAAAAGTAATTAACAAAGGTGTGTGAATGGTGGTGCAGTACATGG
TGTTTGCTTTGTTCA

Last Updated on 06.12.2018 16:11