Sequences from confirmation amplicon


Confirmation sequences for line 787G02 and hit At5g57480 are shown.
Line ID 787G02
Locus At5g57480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23279497
Primers used for PCR 31ew - 8474, show primer details
   
Sequence Name 58-K110987-0022-787-G02C-31ew
GenBank Accession LN509736 [GenBank]
Sequencing primer gene specific primer, 31ew: 5'-TCCCCACATAGAAAAATCTTCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K110987-0022-787-G02C-31ew
TTTTTTTTTGGCAAATCCCCTGGACCCCCCTTTTTAACCACCCTTCTTTTACTTCAAAAC
TTTTCTCATGGAAGCACATTTTGGTTTCTTTCACTTTCAAACTTCCAAGTTTCAAACTTT
CAGGTTTCACCTACTGGTAACACCCAAAAGAAAAAAAAATATCAAACCCTTTGACCATTA
TGTTCTACATTATAAAACTTTAAAAAAAAACAACAAAAACACTTTTCAATCTTCTCTAGT
CTTACAAAGGTTATCCTCAAGCTCAATCTCTTCTTCATCAGGATCCTCACCTTCATTTGG
ACAATCTTTGGCCCTCTTCACAGGCATCTCCCGGGAAATCTACTGGGATCACCAATGAGT
ATGAGGGTCAATATGGAAAAAAAAAAAAAGTAATTCCCAATTTTTTTTCATTTCAAAAAG
GTAAATTTCACCACCGTTTTA
   
Sequence Name 42-K110986-0022-787-G02C-8409
GenBank Accession LN504498 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K110986-0022-787-G02C-8409
CATGTGAGAGGGCAATAGATAGTCAAAATGAAGATGAGGATCATGATGAAGAAGAGATTG
AGCTTGAGGATAACATTTGTAAGACTAGAGAAGATTGAAATGTGTTTTTGTTGTTTTCTT
TTAAAGTTTTATAATGTAGAACATAATGGTCAAAGGTTATGATATTTCTTCTTCTTTTGG
TTGTTACAAGTAGATGAAACATGAAAGTATGAAACTCGGAAGTTAGAAAGTGAAAGAAAC
AAAAATGTGCTACAATGAGAAAAGTTTTGAAGTAAAAAGAAGAGTGATTAAAAAAGGAGG
TTCAAGAGGATTTGGCCAAAAAAAAAAGGGATTTTCAAAAGGCAAATGGTTAATAAAAAT
TTTTCTATGTGGGA

Last Updated on 06.12.2018 16:11