Sequences from confirmation amplicon


Confirmation sequences for line 788C12 and hit At4g12960 are shown.
Line ID 788C12
Locus At4g12960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7583947
Primers used for PCR MG15 - 8474, show primer details
   
Sequence Name 23-K031456-022-788-C12C-MG15
GenBank Accession LN496444 [GenBank]
Sequencing primer gene specific primer, MG15: 5'-ACTGATCTCAAGCTGTTTCCATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K031456-022-788-C12C-MG15
TGACTGTCCTTGCCGGTTCTTTTCATTTCTCCCTCTTCTTGTGGATAAATAAATAAATTT
CCTATCTATATAACTTGTATATTAATGTTATACTTTCTCTTATTAATCATGTTTGGGAAT
GTTGTGAAGCATGGTGAAGAGGAATGCAAACTAAACGCCCTTGAAGCTTGCGCATTAAGA
ACTTGGCCCGATCAGGTATATAAAGAATTGATTTATTTCCTAAGTTTTTTATTCAGGGAA
AACTGAATGTTAGAATTACAGTTTGACAAATGTGATGGTTATGGAACGCAGAAATCACAA
TACTCGTTCATACGGTGCGTCGAAAGCGATACGAAAGGCTGGGAATCATGTGTTAAAAAC
TCTGGACGTGAGAAAGCAATCAATGATTGTTACAATGGTGATCAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
CA
   
Sequence Name 19-K031456-022-788-C12C-8409
GenBank Accession LN494644 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K031456-022-788-C12C-8409
TTACAATTGAATATATCCTGATCACCATTGTAACAATCATTGATTGCTTTCTCACGTCCA
GAGTTTTTAACACATGATTCCCAGCCTTTCGTATCGCTTTCGACGCACCGTATGAACGAG
TATTGTGATTTCTGCGTTCCATAACCATCACATTTGTCAAACTGTAATTCTAACATTCAG
TTTTCCCTGAATAAAAAACTTAGGAAATAAATCAATTCTTTATATACCTGATCGGGCCAA
GTTCTTAATGCGCAAGCTTCAAGGGCGTTTAGTTTGCATTCCTCTTCACCATGCTTCACA
ACATTCCCAAACATGATTAATAAGAGAAAGTATAACATTAATATACAAGTTATATAGATA
GGAAATTTATTTATTTATCCACAAGAAGAGGGAGAAATTGAAAAGAACCTGGCAAGTGAC
AGTCAGATTATCGGAGAGTTCGGCATTACCAAATGGAAACAGCTGAAGATCA

Last Updated on 06.12.2018 16:11