Sequences from confirmation amplicon


Confirmation sequences for line 789A10 and hit At3g54640 are shown.
Line ID 789A10
Locus At3g54640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20225126
Primers used for PCR 16um - 8474, show primer details
   
Sequence Name 11-K117142-0022-789-A10C-16um
GenBank Accession LN491296 [GenBank]
Sequencing primer gene specific primer, 16um: 5'-CCGCAGCCTAGTTGAATAGATAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K117142-0022-789-A10C-16um
GGCAGGGGAGCATAATAAAGGTTGCATTTTGATAGTTGGTTTAAACCTGAATAACAGGTC
CATCAGCTAAAGGGTCAGAGTAAGGAACACCCAATTCGATTATGTCAGAACCACAAGCAT
CAAGAACTTTCAATGCTTCAGCAGTAGTAGAGAGATCCGGATCACCAGCTGTGATATATG
GTATGAATGCTACCTTCACACATAAAAGTTAAACAAATTGCAAATGAACTGTAAATTACA
AAATCCAATCGTATCAGTAAGACCAAGATTGCAAACCTAATCAAACTCAATGCTAACTCA
ATCTGATAAGAAGAATTCATAAGCAATAACAACACATGGTTTTGTGATTCTTTCAAATCC
AAATTTCCCACCAGAACTAACGATAAGTTTTAAAAGTCCAATTCTTGAGGAACGGATTGG
AATATGAAAGACGGAGAATTAACGTACTTTGCCTTGTTTAAGGAAGAAGGATCAGCAATG
AGTATGAAAGACAAATTGGTACTTTAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATATCCG
   
Sequence Name 01-K117141-0022-789-A10C-8409
GenBank Accession LN486748 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K117141-0022-789-A10C-8409
ATTACATTAAAGTACCAATTTGTCTTTCATACTCATTGCTGATCCTTCTTCCTTAAACAA
GGCAAAGTACGTTAATTCTCCGTCTTTCATATTCCAATCCGTTCCTCAAGAATTGGACTT
TTAAAACTTATCGTTAGTTCTGGTGGGAAATTTGGATTTGAAAGAATCACAAAACCATGT
GTTGTTATTGCTTATGAATTCTTCTTATCAGATTGAGTTAGCATTGAGTTTGATTAGGTT
TGCAATCTTGGTCTTACTGATACGATTGGATTTTGTAATTTACAGTTCATTTGCAATTTG
TTTAACTTTTATGTGTGAAGGTAGCATTCATACCATATATCACAGCTGGTGATCCGGATC
TCTCTACTACTGCTGAAGCATTGAAAGTTCTTGATGCTTGTGGTTCTGACATAATCGAAT
TGGGTGTTCCTTACTCTGACCCTTTAGCTGATGGACCTGTTATTCAGGTTTAAACCAACT
ATCAAAATGCAACCTTTATATATGCTTCCCTTGCCTTTTTTTTGTTTGATCATCTTGCTC
TTTGTTATCTATTCAACTAGGCTGCGG

Last Updated on 06.12.2018 16:11