Sequences from confirmation amplicon


Confirmation sequences for line 789A10 and hit At3g54660 are shown.
Line ID 789A10
Locus At3g54660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20232534
Primers used for PCR TK42 - 8474, show primer details
   
Sequence Name 48-K103676-0022-789-A10C-TK42
GenBank Accession LN506484 [GenBank]
Sequencing primer gene specific primer, TK42: 5'-GAGAGAGAGAAGAAGAATCGTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K103676-0022-789-A10C-TK42
GTACATTTCATCATCTTCTCCATCTCTTCAATTCCTCTGCAAAAAACTCCCAATCGCAAT
TCATCTACCATCATCTTCTTCCTCTAGCTTTCTCTCGCTTCCTAAAACCCTAACCTCTCT
CTATTCTCTCCGTCCCCGTATCGCCCTACTCTCAAACCACCGCTATTACCACTCTCGCCG
GTTTTCTGTTTGTGCCAGTACCGATAATGGAGCTGAATCAGACCGCCACTACGATTTTGA
TCTCTTCACTATCGGTGCCGGAAGCGGCGGCGTCCGCGCCTCTCGCTTCGCCACTAGCTT
CGGTGCATCCGCCGCCGTTTGCGAGCTTCCTTTTTCCACTATCTCTTCCGATACTGCTGG
AGGCGTTGGAGGAACGTAAGATTTCTTCCTTCTTGTGGCTTATATCGATAGCTAGTGAAT
TTGACTTTAGTAGTAGCTATACCAGAATTTGAAGCATTTGCTTGATGATTTATGGTTTGA
TTGTGTTGTCTACTCATTTTTGGTTATTTTGCAGGTGTGTATTGAGAGGATGTGTACCAA
ACACTGTGTAATTCTACATGGATCAGCAAAGTACGTTAATTCTCCGTACAAGGCAAAGTA
CCAATTTGGCTTGGAATATATTCATTGGAATGAGTATGTACNAGGCAAAGACGGATTGGA
ATATGAAAGACGGAGAATATGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGT
   
Sequence Name 64-K103677-0022-789-A10C-8409
GenBank Accession LN511184 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K103677-0022-789-A10C-8409
CATTTACATTGAATATATTCATATTCTCCGTCTTTCATATTCCAATCCGTCTTTGCCTTG
TACATACTCATTCCAATGAATATATTCCAAGCCAAATTGGTACTTTGCCTTGTACGGAGA
ATTAACGTACTTTGCTGATCCATGTAGAATTACACAGTGTTTGGTACACATCCTCTCAAT
ACACACCTGCAAAATAACCAAAAATGAGTAGACAACACAATCAAACCATAAATCATCAAG
CAAATGCTTCAAATTCTGGTATAGCTACTACTAAAGTCAAATTCACTAGCTATCGATATA
AGCCACAAGAAGGAAGAAATCTTACGTTCCTCCAACGCCTCCAGCAGTATCGGAAGAGAT
AGTGGAAAAAGGAAGCTCGCAAACGGCGGCGGATGCACCGAAGCTAGTGGCGAAGCGAGA
GGCGCGGACGCCGCCGCTTCCGGCACCGATAGTGAAGAGATCAAAATCGTAGTGGCGGTC
TGATTCAGCTCCATTATCGGTACTGGCACAAACAGAAAACCGGCGAGAGTGGTAATAGCG
GTGGTTTGAGAGTAGGGCGATACGGGGACGGAGAGAATAGAGAGAGGTTAGGGTTTTAGG
AAGCGAGAGAAAGCTAGAGGAAGAAGATGATGGTAGATGAATTGCGATTGGGAGTTTTTT
GCAGAGGAATTGAAGAGATGGAGAAGATGATGAAATTGTACTGGTAAGCTTCGGGGTCGA
AGCCATCTCCACGATTCTTCTTCTCTCT

Last Updated on 06.12.2018 16:11