Sequences from confirmation amplicon


Confirmation sequences for line 791F09 and hit At5g59680 are shown.
Line ID 791F09
Locus At5g59680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24050332
Primers used for PCR EU67 - 8474, show primer details
   
Sequence Name 44-K103737-0022-791-F09C-EU67
GenBank Accession LN505159 [GenBank]
Sequencing primer gene specific primer, EU67: 5'-ACAAATACAAACAGAGCACACGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K103737-0022-791-F09C-EU67
GTATTCGTTGAGTGGGGTTTCTTACAATTGTGTCACTTGCAGCACAAACTATCTTGCTCT
GGGAATCATCTCAGCATCAAAGATCATGCTCACTTCAGGGGAACTTAGTGTATCCATGCC
TCGACTCATATTTCTCCTCGAGTTTTCAGACACGAGACACTCTTTTAGTTCAATAACAAC
ATGAGACATGGTTGGTCGTCTTGCTGAGGTAGGATCCGCGCACGACATTGCTAGCTCAAG
AGCTCTCCACGCAGAACGAGAATCATAATCACCGTTAAGCTTTAGATCCATGATCTTAGC
AATATCTCCTCCGTTAAGCTCAGACCCTACCCATTGTGTTATGTGAGACTTTCTGCGGTT
TCGATCAATCACTGCTTGGTTTGTGATCATCTCTAATAAAACAATCCCGAAACTGTAAAC
ATCACTTTTCTCACTCAACCGACTTGTATGGTAATATCTACAAGGAAAGCGAATGGTATA
TACTCAATTGATTCATGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATATC
   
Sequence Name 60-K103738-0022-791-F09C-8409
GenBank Accession LN510240 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K103738-0022-791-F09C-8409
GCCTTTCTGAATCAATTGAGTATATACCATTCGCTTTCCTTGTAGATATTACCATACAAG
TCGGTTGAGTGAGAAAAGTGATGTTTACAGTTTCGGGATTGTTTTATTAGAGATGATCAC
AAACCAAGCAGTGATTGATCGAAACCGCAGAAAGTCTCACATAACACAATGGGTAGGGTC
TGAGCTTAACGGAGGAGATATTGCTAAGATCATGGATCTAAAGCTTAACGGTGATTATGA
TTCTCGTTCTGCGTGGAGAGCTCTTGAGCTAGCAATGTCGTGCGCGGATCCTACCTCAGC
AAGACGACCAACCATGTCTCATGTTGTTATTGAACTAAAAGAGTGTCTCGTGTCTGAAAA
CTCGAGGAGAAATATGAGTCGAGGCATGGATACACTAAGTTCCCCTGAAGTGAGCATGAT
CTTTGATGCTGAGATGATTCCCAGAGCAAGATAGTTTGTGCTGCAAGTGACACAATTGTA
ATGAAACCACCACTCAACGAATTTACTTGTGGCTTTGACATGTCGTGTGCTCTGTTGNTA
TTT
Primers used for PCR 77wp - 3144, show primer details
   
Sequence Name 31-K118742-0022-791-F09C-77wp
GenBank Accession LN500351 [GenBank]
Sequencing primer gene specific primer, 77wp: 5'-AAGGCAGAGGTATTTCCAGTACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K118742-0022-791-F09C-77wp
ACCTAATGGGTTTTTTTGTTTTCCTTTGCATGAATGCATTAGGTTGATCTTCTTCTGAGA
GTTCACCATACGAATTTGGTAAGCCTTGTCGGATATTGTGATGAAGGAGACCACTTGGCC
CTTATCTACGAGTTTGTACCCAATGGAGACTTAAGACAACATTTGTCTGGTAAAACTTGA
TGTAACATTTTATAATGGTTATAATATGTATCATGAACTCATGAAAACATGAAAGGATCT
AATGGTCTGATATATAGGAAAAGGAGGTAAACCCATCGTCAATTGGGGTACTCGGCTAAG
AATAGCGGCGGAGGCAGCACTAGGTTTGTACCAAGTTACACTGTAGAACTTGAATCCATT
CGATTGGTTTTACAAGAATCATTTAATATTATTGAATCATTTTGCATAGGTTTGGAGTAC
TTACACATTGGATGCACACCACCAATGGTTCATAGAGATGTCAAAACTACAAACATATTG
CTGGACGAGCATTATAAGGCCAAACTTGCTGATTTTGGGCTCTCGAGATCTTTCCCTGTT
GGAGGTGAATCTCATGTTTCGACGGTGATTGCTGGTACTCCTGGTTACCTTGATCCAGAG
TAAGAATCAATTAACAGTGCTACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCT
GATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTC
TTGCGAAGGATAGTGGGATTGTGCGTCATCCCATACATCAGTGGAGATATCACATCAATC
   
Sequence Name 15-K118741-0022-791-F09C-35St
GenBank Accession LN493193 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K118741-0022-791-F09C-35St
ATCCACTTCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTAGCAC
TGTTAATTGATTCTTACTCTGGATCAAGGTAACCAGGAGTACCAGCAATCACCGTCGAAA
CATGAGATTCACCTCCAACAGGGAAAGATCTCGAGAGCCCAAAATCAGCAAGTTTGGCCT
TATAATGCTCGTCCAGCAATATGTTTGTAGTTTTGACATCTCTATGAACCATTGGTGGTG
TGCATCCAATGTGTAAGTACTCCAAACCTATGCAAAATGATTCAATAATATTAAATGATT
CTTGTAAAACCAATCGAATGGATTCAAGTTCTACAGTGTAACTTGGTACAAACCTAGTGC
TGCCTCCGCCGCTATTCTTAGCCGAGTACCCCAATTGACGATGGGTTTACCTCCTTTTCC
TATATATCAGACCATTAGATCCTTTCATGTTTTCATGAGTTCATGATACATATTATAACC
ATTATAAAATGTTACATCAAGTTTTACCAGACAAATGTTGTCTTAAGTCTCCATTGGGTA
CAAACTCGTAGATAAGGGCCAAGTGGTCTCCTTCATCACAATATCCGACAAGGCTTACCA
AATTCGTATGGTGAACTCTCAGAAGAAGATCAACCTAATGCATTCATGCAAAGGAAAACA
AAAAAACCCATTAGGTTTCTGATGTTGAAAACAGATACTAGTTAGAAAGATTGTACTGGA
A

Last Updated on 06.12.2018 16:11