Sequences from confirmation amplicon


Confirmation sequences for line 791G05 and hit At1g15270 are shown.
Line ID 791G05
Locus At1g15270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5250905
Primers used for PCR 01dp - 8474, show primer details
   
Sequence Name 17-K109642-0022-791-G05C-01dp
GenBank Accession LN493890 [GenBank]
Sequencing primer gene specific primer, 01dp: 5'-TTTGATGCTCTGACTTGTTTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K109642-0022-791-G05C-01dp
AAATGCTCTAGTTAACTTATATGTTTGGGCTCCTCTTTCAATGGAAAGCTATAAAATACG
AATCAAAGATTGTATCTTCACAAGAGAGCTTCTGTTTAGATACATAGGTTTGTAAAGGTT
TTGTAAAGGTTGTGCATTAATATGTTCCACTTTGTCAGTTTGGTCTACTAATCAAGCTGA
CTCTAATTATGACTTTATAGCTGGTGACTACAACTAGATGGGTCATTTCTATTAATTATT
TGTTAGTTTAGGGGTATTCATTACAGATTCTTTTGAGATGATATGGACTAGTTTCTTCTT
ACTTTGATGAAATAGTGACCATTTGCCCATGTTAGGATGTTTAGTCTACTTTTTTTCCAC
CCAATTGTGTGAAAGACTCATTAGCCTTTCTTTCGCATGAGAAGTACAGTGATCATTTGT
TACCGAAAGCCTGACCTGTTTTGTTTTGAATGAAAATGATAAAACAGGCCCTTAAAAAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGC
   
Sequence Name 01-K109633-0022-791-G05C-8409
GenBank Accession LN486614 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K109633-0022-791-G05C-8409
TTGAATATATCCTTTTTAAGGGCCTGTTTTATCATTTTCATTCAAACAAAACAGGTCAGG
CTTTCGGTAACAAATGATCACTGTACTTCTCATGCGAAAGAAAGGCTAATGAGTCTTTCA
CACAATTGGGTGGAAAAAAAGTAGACTAAACATCCTAACATGGGCAAATGGTCACTATTT
CATCAAAGTAAGAAGAAACTAGTCCATATCATCTCAAAAGAATCTGTAATGAATACCCCT
AAACTAACAAATAATTAATAGAAATGACCCATCTAGTTGTAGTCACCAGCTATAAAGTCA
TAATTAGAGTCAGCTTGATTAGTAGACCAAACTGACAAAGTGGAACATATTAATGCACAA
CCTTTACAAAACCTTTACAAACCTATGTATCTAAACAGAAGCTCTCTTGTGAAGATACAA
TCTTTGATTCGTATTTTATAGCTTTCCATTGAAAGAGGAGCCCAAACATATAAGTTTAAC
TAGATGCATTTTTCCAATCACAAAACTTCATATGAACAAAAAGAGAAAACAAGTCAGAGC
ATCAA

Last Updated on 06.12.2018 16:11