Sequences from confirmation amplicon


Confirmation sequences for line 791H08 and hit At5g59450 are shown.
Line ID 791H08
Locus At5g59450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23974927
Primers used for PCR CP48 - 8474, show primer details
   
Sequence Name 19-K031183-022-791-H08C-8409
GenBank Accession LN494642 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K031183-022-791-H08C-8409
CGGAATGAGCCTTTACATTGAATATATCCGGACATGGATCTTTCTTTTTCTTCACCAAAC
GATTCACCTCCAACGGTGTGTTTGGATAACTCTCCTGTTCTGAAGTACATCAATGATATG
TTGATGGACGAAGAAGACTTCGTAGGTATATCTCGTGACGATTTAGCTTTACAAGCCGCT
GAGAGATCCTTCTACGAGATAATTCAACAGCAATCTCCAGAATCTGATCAGAACACTAGC
TCCTCCTCCGATCAAAACTCCGGTGACCAAGATTTCTGTTTTCCCAGCACAACAACAGAT
TCATCAGCGTTGGTTTCGAGCGGAGAATCACAGAGAAAGTACCGTCATCGGAATGATGAA
GAAGATGATCTTGAGAACAATCGGAGAAACAAACAACCAGCGATATTTGTCTCGGAANGG
GGAAGAG
   
Sequence Name 20-K031183-022-791-H08C-CP48
GenBank Accession LN495096 [GenBank]
Sequencing primer gene specific primer, CP48: 5'-ACTCTTCCATCTCCGAGACAAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K031183-022-791-H08C-CP48
AGATCATCTTCTTCATCATTCCGATGACGGTACTTTCTCTGTGATTCTCCGCTCGAAACC
AACGCTGATGAATCTGTTGTTGTGCTGGGAAAACAGAAATCTTGGTCACCGGAGTTTTGA
TCGGAGGAGGAGCTAGTGTTCTGATCAGATTCTGGAGATTGCTGTTGAATTATCTCGTAG
AAGGATCTCTCAGCGGCTTGTAAAGCTAAATCGTCACGAGATATACCTACGAAGTCTTCT
TCGTCCATCAACATATCATTGATGTACTTCAGAACAGGAGAGTTATCCAAACACACCGTT
GGAGGTGAATCGTTTGGTGAAGAAAAAGAAAGATCCATGTCCGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
Primers used for PCR 19au - 8474, show primer details
   
Sequence Name 83-K118414-0022-791-H08C-19au
GenBank Accession LN514355 [GenBank]
Sequencing primer gene specific primer, 19au: 5'-GTAGTTGCAGATAGATCCGACACA-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K118414-0022-791-H08C-19au
TTTTATAACTTGATCCACTTTTTGTGGCTGCTTACTGTTTTCCTCGAAAGTTCCATTTTT
TTCTTTTGCTCTGCAAACGAAAAAGAACCATAAATTTTTCTCATATTTAAAGCTCTCTCT
TTCTCTCACAACTGTGTTCACATGAACTTTCTCACCGCTTTCTTCTCTTCATTGAACAAA
AAAAAAAGCTCTAAACTTTTTTTTCTTCTTTAGGTAAAATTTGACAAAAGCTCTCAACTT
TCAAACTCTGTTTCTTCATCATCATCATCATCACCATGGACGCCCTGCTTCAAGTTTCAG
TTGATGGGTTTAAATTTGAAAACGGATCTGGATCTTGCTGCAAACCCAAAAACAATCTCA
AATCTGGAAACAATCTCTTCCCTGATTTCCACAAGTATATTCAATTGTAAATGGCTTCAT
GTCCGGAAAATCTACATGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAAAAAA
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATTTCC
   
Sequence Name 35-K118413-0022-791-H08C-8409
GenBank Accession LN502006 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K118413-0022-791-H08C-8409
ATTTCATTGAATATCTCGTGGAAACAGGGAAGAGATTGTTCCCAGATTCGAGATTGTTTC
TGGGTTTGCAGCAAGATCCAGATCCGTTCTCAAATCTAAACCCATCAACTGAAACTTGAA
GCAGGGCGTCCATGGTGATGATGATGATGATGAAGAAACAGAGTTTGAAAGTTGAGAGCT
TTTGTCAAATTTTACCTAAAGAAGAAAAAAAAGTTAAGAGCTTTTTTTTTTGTTCAATGA
AAAGAAAAAAGCGGTGAGAAAGTTCATGTGAACACAGTTGTGAGAGAAAGAGAGAGCTTT
AAATATGAAAAAAATTTATGGTTCTTTTTCGTTTGCAAAGCAAAAAAAAAAAATGGAACT
TTCGAGGAAAACAGTAAGCAGCCACAAAAAGTGGATCAGGTTATTAAAACATACTTAATT
TAAAAGGTTAGTTAATTTGTGTCGAATCTA

Last Updated on 06.12.2018 16:11