Genotyping and primer details


Genotyping and primer details for 791H08 - At5g59450
Line ID 791H08
Locus At5g59450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23974927 of line 791H08 Chr5:23974927
   
Insertion allele amplicon 8474 - 19au
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19au: 5'-GTAGTTGCAGATAGATCCGACACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23974480
   
Insertion allele amplicon 8474 - CP48
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CP48: 5'-ACTCTTCCATCTCCGAGACAAATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23975318
   
wt amplicon (over InsPos) CP48 - 19au
wt amplicon start Chr5:23974480
wt amplicon end Chr5:23975318
wt amplicon size 839 bp
Locus specific primer 19au: 5'-GTAGTTGCAGATAGATCCGACACA-3'
2nd locus specific primer CP48: 5'-ACTCTTCCATCTCCGAGACAAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11