Sequences from confirmation amplicon


Confirmation sequences for line 794E08 and hit At5g50400 are shown.
Line ID 794E08
Locus At5g50400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20524977
Primers used for PCR MK10 - 8474, show primer details
   
Sequence Name 49-K030903-022-794-E08C-8409
GenBank Accession LN506730 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K030903-022-794-E08C-8409
CATTTACAATTGAATATATCCTGGGATCACGCCAACAGTACGAGCAGGAGCATAAGAGTC
AAACAGTGTAGTAAGAATCAAAATGCAAATTCCCATAAAGAGAAAAGTGCATAGATTTCT
AGTTTCATACATACCACACATGCTGTTCCTGGTAAAAGTCAAGGTACCAGCTGGCGATCG
TCTACTTCGAGTTCCCTTACGACTCCATTCCACAAACGGAACAGCCTCACCTATGTTGTA
ACCACTTGTCCATGTTACGGTCATCTATCAAAATCATAGTGCAAAGCATGTTTTAGTTCA
TATATCGATTTGAGAGTCTTGTTAAGGCTTTGATAAATCAGGCTATGGAAAACTTACCTC
GTCCCATTTCTTCCCTAAAGCAAGACGTGGATAGACAGGAGCTTTAGGATGGAATAAAC
   
Sequence Name 50-K030903-022-794-E08C-MK10
GenBank Accession LN507062 [GenBank]
Sequencing primer gene specific primer, MK10: 5'-CGTTTATCAATCCTAAAGCTCCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K030903-022-794-E08C-MK10
AGCCTGATTTATCAAAGCCTTAACACGACACACAAATCGATATATGAACTAAAACATGCT
TTGCACTATCGATTTTTGATAGATGACCGTAACATGGACAAGTGGTTACAACATAGGTGA
GGCTGTTCCGTTTGTGGAATGGAGTCGTAAGGGAACTCGAAGTAGACGATCGCCAGCTGG
TACCTTGACTTTTACCAGGAACAGCATGTGTGGTATGTATGAAACTAGAAATCTATGCAC
TTTTCTCTTTATCTGAATTTGCATTTTGATTCTTACTACACTGTTTGACACAGGTGCTCC
TGCTCGTACTGTTGGCGTGATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11