Sequences from confirmation amplicon


Confirmation sequences for line 794E12 and hit At3g15810 are shown.
Line ID 794E12
Locus At3g15810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5348467
Primers used for PCR 40vf - 8474, show primer details
   
Sequence Name 32-K117753-0022-794-E12C-40vf
GenBank Accession LN500745 [GenBank]
Sequencing primer gene specific primer, 40vf: 5'-GGTGATATCATCTTCAGGGTTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K117753-0022-794-E12C-40vf
CTCATGGACGCGACCGGGAAGTGTCTCCTTACCGTTAAACGAAAGGTAAACTTAATTCAT
CCTTTTTTTCTTCTTAAAATTATGTTGGGATGAGTAAGAGTCTAGTCGTGAGGGTGACTA
GCTAGGTGATTATAATAAATTTTGATGCTCAACTTCTTGTGCTGAATTTGATTAGATGGG
TTTGGTTTAAAAAATGAATTTCATTGGTGAGCATCCATCAGAATTCAAGAATCTTTTGCT
AACCTCTTTTTTTTGTCAAGAAAATCTTGATAAGTTTTTTGTATTGGTAAACAAATAATG
ACTTGGCTAGAATCGATAAAAATCATTGTTAGAAACGAGATCTGAAGAAATTGTTTTGAG
CTGAAAATTTTAATCAACTTTTTGTATAAGCCTAATTCCGAGTCATCGACTCAGTTGCTA
ACACAATTTATAGTGTCCTTTATTTGTTATATAGTAGTTCTAAAGATGATACTGAGTCAA
CTCGTTGTTTTATTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAG
   
Sequence Name 16-K117752-0022-794-E12C-8409
GenBank Accession LN493611 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K117752-0022-794-E12C-8409
AAAAACAACGAGTTGACTCAGTATCATCTTTAGAACTACTATATAACAAATAAAGGACAC
TATAAATTGTGTTAGCAACTGAGTCGATGACTCGGAATTAGGCTTATACAAAAAGTTGAT
TAAAATTTTCAGCTCAAAACAATTTCTTCAGATCTCGTTTCTAACAATGATTTTTATCGA
TTCTAGCCAAGTCATTATTTGTTTACCAATACAAAAAACTTATCAAGATTTTCTTGACAA
AAAAAAGAGGTTAGCAAAAGATTCTTGAATTCTGATGGATGCTCACCAATGAAATTCATT
TTTTAAACCAAACCCATCTAATCAAATTCAGCACAAGAAGTTGAGCATCAAAATTTATTA
TAATCACCTAGCTAGTCACCCTCACGACTAGACTCTTACTCATCCCAACATAATTTTAAG
AAGAAAAAAAGGATGAATTAAGTTTACCTTTCGTTTAACGGTAAGGAGACACTTCCCGGT
CGCGTCCATGAGGACAATCTCATCGTTATCTCTGGTGTCAGGTCCATAAGAATCAACCCT
G

Last Updated on 06.12.2018 16:11