Sequences from confirmation amplicon


Confirmation sequences for line 796C12 and hit At2g17860 are shown.
Line ID 796C12
Locus At2g17860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7762784
Primers used for PCR NF32 - 8474, show primer details
   
Sequence Name 07-K102588-0022-796-C12C-8409
GenBank Accession LN489221 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K102588-0022-796-C12C-8409
GAGCCTTTACAATTGAATATATCCTGACCAATTTACATGAAGCCATTTACATGTAGAGCG
TGCGTCCCCATATGCGGCCGGACCAAGCGGCGGGTATGGAGATGAGTCGTGACTCAAAGG
AGTTGAGGGAGAAACCAGTGGTGGGTAATGGAGCGGTGCCGGCGCCGGAGAGAAGTCCCG
GCCAGACGGTGTAGTTACACTGGTTAACGATGGTGAACGTGGTGGAGGAACCACCATTGA
TGAAAAACATAATCGCTAAGAGAAAGCAAGAGACTTTAGCCAAATCCATTGTTACCGTTT
GATGTTGTATGATTCAGGAGAAGTTAGGGATCGTGTTTATATAAGAGAATCGGGGATTTG
TGTTATGGAAAGTTTCATAATATCAATTTTCCCTATATGAGTTTTGCCATTACAGAATCC
GATACATGTTCACTCATCATTTCCTTTATTTCAATATTCTCATTAATACTTGTGAAATGG
AAATTACCGAATC
   
Sequence Name 07-K102589-0022-796-C12C-NF32
GenBank Accession LN489222 [GenBank]
Sequencing primer gene specific primer, NF32: 5'-TGATTCGTAATTTCCATTTCACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K102589-0022-796-C12C-NF32
GATAAGGAATGATGAGTGAACATGTATCGGATTCTGTAATGGCAAAACTCATATAGGGAA
AATTGATATTATGAAACTTTCCATAACACAAATCCCCGATTCTCTTATATAAACACGATC
CCTAACTTCTCCTGAATCATACAACATCAAACGGTAACAATGGATTTGGCTAAAGTCTCT
TGCTTTCTCTTAGCGATTATGTTTTTCATCAATGGTGGTTCCTCCACCACGTTCACCATC
GTTAACCAGTGTAACTACACCGTCTGGCCGGGACTTCTCTCCGGCGCCGGCACCGCTCCA
TTACCCACCACTGGTTTCTCCCTCAACTCCTTTGAGTCACGACTCATCTCCATACCCGCC
GCTTGGTCCGGCCGCATATGGGGACGCACGCTCTACATGTAAATGGCTTCATGTAAATTG
GTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACATTCAC

Last Updated on 06.12.2018 16:11