Sequences from confirmation amplicon


Confirmation sequences for line 796F06 and hit At1g12000 are shown.
Line ID 796F06
Locus At1g12000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4052591
Primers used for PCR 35ve - 8474, show primer details
   
Sequence Name 32-K117967-0022-796-F06C-35ve
GenBank Accession LN500750 [GenBank]
Sequencing primer gene specific primer, 35ve: 5'-AATAGGGCACAATAGAAATGATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K117967-0022-796-F06C-35ve
TTACTTCATGATAGTACTTTCCGTCGACCTTGCATCAATCATGACGTTTCCGATCATTTC
AGAGTAAATCTGCATAGGCATTAAAATGTTTTTAATAGGCTTTTTCCAACATAATTCTTC
TGGTGGAACAAGAAATTAGATTCTTGACGAGGAATTGAAACGAGAGCAAAGGATTGGTTA
TGTAATAACTTATGTAACAATTGATAAAAAAAAAAAAAATGATCAAAACGGAATATCCCC
TTGCAGGCTGTTTCAACCCCAAAACTAGTGGGAACCTTTTTGCTTTTAAAACCCCCATCA
AGGGTTTGGGGGCCCCCAAGGACCCGGGTTTTCAAGTTCTACCCCCTAATTATACAGGTA
AATAAAAAGCCAAAGGCTGAATCCCCAAATTTAAAAAATTCGGGTCCCAGGGATTTTCAA
AAACAACTACTTTCCCCAAAAAAAAAAAATTTTCAACAAAACAAATTCAACTGCAGGGTT
TCCCTGAATTTTTCCCCAAAAAGGCAACCTTTGGTTTTGAAATCTTCCCCCCCGATACCC
ACCATTTGAAAAGGGTTTCTTGTCCGGGAAATCTACGGGGATCACCAAGGAGTATGAGGG
TCAATTGGGAAAAAAAAAAAAAGTATTTCCCATTTTTTTTTCATTTCAAAAATGTAAATT
TCCCC
   
Sequence Name 16-K117966-0022-796-F06C-8409
GenBank Accession LN493618 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K117966-0022-796-F06C-8409
ATTTCAATTGGTGGTTATCGGTGGAGATGATTCCACACCAATGCTTGCCTTCTTGCTGAA
AACTTCAGGTAAACCTTGCAGTTGAATCTGTTATGTTGAAACTTATTTATCTTAGGCGAA
AGTAGTTGTTCTTGAAGATCCATGTGACCAGAATCTTTAATATTTGCTGATTCAGCATTT
GACTTATTATCTACTTGTATAATTAGGAGTAAGAACTTGAAAACCCGAGTCATTGGGTGC
CCCAAGACCATTGATGGTGATTTGAAATGCAAAGAGGTTCCTACTAGTTTTGGGTTTGAT
ACAGCTTGCAAGGTGATATTCCGTTTTGATCATTTTTTTTTTTTTTACCATTTGTTCCAA
AATTTTTTACAAACCCAACCCTTTGCTCTCTTTTAAATTCCCCGCAAAAAATCAAATTTC
TTGTTCCCCCAAAAAAATTTTTTTGGAAAAACCCTATAAAAAACTTTTTAAGGCCTATGC
AAATTTCCCCTAAAAGGATCGAAAACTTCTTGATTGATGCAGGGCCAACGGGAAAGTACT
ATCTTTGAAAGTAAATGTTTAACAAAATTTCTGGAATTTGCACCATTTCTTG

Last Updated on 06.12.2018 16:11