Sequences from confirmation amplicon


Confirmation sequences for line 797D11 and hit At3g57787 are shown.
Line ID 797D11
Locus At3g57787 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21405018
Primers used for PCR CT27 - 8474, show primer details
   
Sequence Name 81-K105064-0022-797-D11C-CT27
GenBank Accession LN514071 [GenBank]
Sequencing primer gene specific primer, CT27: 5'-TGATTACGTTATGGTTGGTGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K105064-0022-797-D11C-CT27
GATTCTTATATTTCTTTGTGAGAGAATTGGAAATTTGGTTTTTGTTTTCTCCAAAATGAC
TACGTTGTGAATTGTGTGAGCACGAGTTCTCCAACGACACCGTTTTCAACAAGTAATTGT
AGGTTTTCTCACACTAGAGTTTATTTACGTCTAATCATCAATATATCACTGAGAGTGAAA
TCGGAATCACAAAAATGGCGTCGACGAAGCCATTGATTGTCGCAGCGAAGACGCTCCGAA
ACCGGATCCACTTCCGATCTGGATCCACATCTACTGGTCCAAGCCGGTGGGCGACACCGG
GTCACGAAGAACGACCCAAAGGATATTTCATGAACCGGACTCCGCCGCCTCCAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATT
   
Sequence Name 65-K105065-0022-797-D11C-8409
GenBank Accession LN511399 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K105065-0022-797-D11C-8409
CATTTCATTGAATATATCCTGGAGGCGGCGGAGTCCGGTTCATGAAATATCCTTTGGGTC
GTTCTTCGTGACCCGGTGTCGCCCACCGGCTTGGACCAGTAGATGTGGATCCAGATCGGA
AGTGGATCCGGTTTCGGAGCGTCTTCGCTGCGACAATCAATGGCTTCGTCGACGCCATTT
TTGTGATTCCGATTTCACTCTCAGTGATATATTGATGATTAGACGTAAATAAACTCTAGT
GTGAGAAAACCTACAATTACTTGTTGAAAACGGTGTCGTTGGAGAACTCGTGCTCACACA
ATTCACAACGTAGTCATTTTGGAGAAAACAAAAACCAAATTTCCAATTCTCTCACAAAGA
AATATAAGAATCCAAAATCTCTTTACTAAGCAAAAGCAATGTTGTTTTCACCAACCATAN
CGTAATC

Last Updated on 06.12.2018 16:11