Sequences from confirmation amplicon


Confirmation sequences for line 797G07 and hit At5g36000 are shown.
Line ID 797G07
Locus At5g36000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14140535
Primers used for PCR GA54 - 8474, show primer details
   
Sequence Name 12-K101382-0022-797-G07C-8409
GenBank Accession LN491509 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K101382-0022-797-G07C-8409
TTTACATTGAATAATCCTGATTTGTCAGAGAAAATTAGATCTAAGAAATCTGATTCGAAA
TTGAATTTTCAAAACTCAAATCCCAGGTTTCAGCAACCACACAGGTCACGAACAGGGATC
GATATAAGCATCTAATTCTCATACAAATCAAATTGATTCCAGAAGGTATCAAAGATGATT
GATCGAACTCTATTTCCTAGATCCATCCAATACATGCCGTACAAATCAAATTCGAGCTCA
GCCCAAACTCGCACACCAAAAATATCTGAGAACAGATCTATGTAGTTCATAGAGGAGAAG
AAAATACCAGGAGAAGCGAGACTGGTAAGTCGGAGCAGAGCACCGAGGAGATACACAGCC
ATGGATTCCTCTCAGGATCTTCCCGTTCTTCTGGTCGCTGCTCTGAAT
   
Sequence Name 12-K101388-0022-797-G07C-GA54
GenBank Accession LN491510 [GenBank]
Sequencing primer gene specific primer, GA54: 5'-AAGAGAAGAATCATTCAGAGCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K101388-0022-797-G07C-GA54
ATCCATGGCTGTGTATCTCCTCGGTGCTCTGCTCCGACTTACCAGTCTCGCTTCTCCTGG
TATTTTCTTCTCCTCTATGAACTACATAGATCTGTTCTCAGATATTTTTGGTGTGCGAGT
TTGGGCTGAGCTCGAATTTGATTTGTACGGCATGTATTGGATGGATCTAGGAAATAGAGT
TCGATCAATCATCTTTGATACCTTCTGGAATCAATTTGATTTGTATGAGAATTAGATGCT
TATATCGATCCCTGTTCGTGACCTGTGTGGTTGCTGAAACCTGGGATTTGAGTTTTGAAA
ATTCAATTTCGAATCAGATTTCTTAGATCTAATTTTCTCTGACAAATCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CA

Last Updated on 06.12.2018 16:11