Sequences from confirmation amplicon


Confirmation sequences for line 799C01 and hit At2g31200 are shown.
Line ID 799C01
Locus At2g31200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13294561
Primers used for PCR 84qa - 8474, show primer details
   
Sequence Name 35-K115495-0022-799-C01C-84qa
GenBank Accession LN501952 [GenBank]
Sequencing primer gene specific primer, 84qa: 5'-TTTGTTTGATAAGTTCGTGTCGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K115495-0022-799-C01C-84qa
GAAGCAGAATCAATCCATGTCTTTCAGAGGACTTAGCAGGGTAAAATTACAAAAAAAAAC
TTTTCTCTTCTTGCTTAAAATCGATGTACTCTTGTCTATGTCGCAAGCATCTTCAATGTT
AAAAATTTGATTTTTATGAAGTCGATGTTAAAAAAAACTAAACCCATCTCCATTTTTACT
CTTATTGAGGGTTTAAGATCACTAGATGAAGTCAATTCGATGAATTTTGGATTAAAAATC
GAATCTTTTGGTATGGTTTTGATATGTGTTTTGGTTTTATATCTGTAGCCAAATGCAATA
TCTGGAATGGGTGTTGCAGATGAGAGCAAAACCACATTTCTAGAGCTTCAAAGGAAAAAA
ACTCATCGCTATGTGGTCTTCAAGATTGATGAATCCAAAAAAGAAGTTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 24-K115494-0022-799-C01C-8409
GenBank Accession LN497126 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K115494-0022-799-C01C-8409
ACTTCTTTTTTGGATTCATCAATCTTGAAGACCACATAGCGATGAGTTTTTTTCCTTTGA
AGCTCTAGAAATGTGGTTTTGCTCTCATCTGCAACACCCATTCCAGATATTGCATTTGGC
TACAGATATAAAACCAAAACACATATCAAAACCATACCAAAAGATTCGATTTTTAATCCA
AAATTCATCGAATTGACTTCATCTAGTGATCTTAAACCCTCAATAAGAGTAAAAATGGAG
ATGGGTTTAGTTTTTTTTAACATCGACTTCATAAAAATCAAATTTTTAACATTGAAGATG
CTTGCGACATAGACAAGAGTACATCGATTTTAAGCAAGAAGAGAAAAGTTTTTTTTTGTA
ATTTTACCCTGCTAAGTCCTCTGAAAGACATGGATTTGATTCTGCTTTCTTCAGACACCA
CAGAATTGGGTTAGAGAGACGAAGACGACACGA

Last Updated on 06.12.2018 16:11