Sequences from confirmation amplicon


Confirmation sequences for line 800B03 and hit At1g64295 are shown.
Line ID 800B03
Locus At1g64295 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23860231
Primers used for PCR 24vi - 8474, show primer details
   
Sequence Name 67-K117751-0022-800-B03C-24vi
GenBank Accession LN511897 [GenBank]
Sequencing primer gene specific primer, 24vi: 5'-ATGCTCTACTCCAATGCTTTCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K117751-0022-800-B03C-24vi
CTCTTTAAACCCATAATCTGGGAACATTCTTTCTTGATTGTTGTTTATCCCAGAGACGAA
TAACACCTAGCTTCCCTTCGTAGCTGACTAACTCTGGGAATAAACCAACGTTTGGAGTAT
GTAAGAAAGACCAAGTTTCGGTTTTGGGACATAACCGGATCACATTGTGGTTCCATGATC
TCCAATGCAAGAACCCTGAAGCTTGTACCGGGTTTGAAAGAATTAGACCGTCTGTTCTCG
GTAGACGCAAATTTTTTAATCTCTTCCACATAAACGAATCGGAATCAAAAACTTCGCACG
CAAAAGTTCTGAGATTCCGAGTCATACCAGGCGATTTCGAAAGCCTAAATATTTTATACC
GGAAAGGCTGCAATCCGGTTACCGTTAGACCTAATGACCAATGACGCAATGAGTATATTT
TGCAAATGTAAATGATCCATATGGAGAACATGAATCAGCAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTC
   
Sequence Name 42-K117750-0022-800-B03C-8409
GenBank Accession LN504621 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117750-0022-800-B03C-8409
TCCCTGCTGATTCATGTTCTCCATATGGATCATTTACATTTGCAAAATATACTCATTGCG
TCATTGGTCATTAGGTCTAACGGTAACCGGATTGCAGCCTTTCCGGTATAAAATATTTAG
GCTTTCGAAATCGCCTGGTATGACTCGGAATCTCAGAACTTTTGCGTGCGAAGTTTTTGA
TTCCGATTCGTTTATGTGGAAGAGATTAAAAAATTTGCGTCTACCGAGAACAGACGGTCT
AATTCTTTCAAACCCGGTACAAGCTTCAGGGTTCTTGCATTGGAGATCATGGAACCACAA
TGTGATCCGGTTATGTCCCAAAACCGAAACTTGGTCTTTCTTACATACTCCAAACGTTGG
TTTATTCCCAGAGTTAGTCAGCTACGAAGGGAAGCTAGGTGTTATTCGTCACTGGATAAA
CAACAATCAAGAAGATGTTCACAGATTATGGGTTTTAAAGAGCAGTTTTGAAAAGTCGTG
GGAAAAAGTGAAAGATATTGAAAGCATTG
Primers used for PCR 65xd - 8474, show primer details
   
Sequence Name 22-K118953-0022-800-B03C-65xd
GenBank Accession LN496333 [GenBank]
Sequencing primer gene specific primer, 65xd: 5'-TATATTTTTATCTGGGACTGGCGA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K118953-0022-800-B03C-65xd
TCTCTTTCTTTCTTTTTTCCTATAAGATCATGAGTTCTACGGTGATAAATCAGGACACGA
TCCAAGAGGTTCTTTCTTATCTTCGGGGGTCCGAAATCGGAAAATTGAGGCTCATCAATA
AAGAGTGCAACAAGAGAAGCTACGAGTCTTGGTTTGTCAACCTAAATCTCCATCGAACCA
ACAATATCTCTGGATACTTCCTAGAACGCTACGAAAGAGGATACAAACTTGATACCAGTT
TTTTCCATGAGAGGAGAGATTTGGATAACAAAGGAGTCTCCATCGATTTTCTGCCACAAG
GAGAGGTAAAGATCAAAGCTTGTGATGCACGTCATGGGATATTGCTTTGTTATAATGATA
CAAGACCAATACCGGAATACATAGTCTGCAAACCAACAACAAAACAGTATCAGATCATAC
CAATTGTAACCGGATTGCAATTAAATATACATGAAGAAAATACCGCATCAGGCGCCATTC
GCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACG
CCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTC
CCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTTGCATGTCGACATGCATGCAT
GTCGACATGCATGCATGTCGACTCTAGAGGATCCCCAATTCCCATGGAGTCAAAGATTCA
AATAGAG
   
Sequence Name 06-K118952-0022-800-B03C-8409
GenBank Accession LN489027 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K118952-0022-800-B03C-8409
TCAGCGTGTCGTGCTCCACCATGTTGACGAAGATTTTCTTCTTGTCATTGAGTCGTAAGA
GACTCTGTATGAACTGTTCGCCAGTCTTTACGGCGAGTTCTGTTAGGTCCTCTATTTGAA
TCTTTGACTCCATGGGAATTGGGGATCCTCTAGAGTCGACATGCATGCATGTCGACATGC
ATGCATGTCGACATGCAAGCTTGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAA
ACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTA
ATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAAT
GGCGCCTGATGCGGTATTTTCTTCATGTATATTTAATTGCAATCCGGTTACAATTGGTAT
GATCTGATACTGTTTTGTTGTTGGTTTGCAGACTATGTATTCCGGTATTGGTCTTGTATC
ATTATAACAAAGCAATATCCCATGACGTGCATCACAAGCTTTGATCTTTACCTCTCCTTG
TGGCAGAAAATCGATGGAGACTCCTTTGTTATCCAAATCTCTCCTCTCATGGAAAAAACT
GGTATCAAGTTTGTATCCTCTTTCGTAGCGTTCTAGGAAGTATCCAGAGATATTGTTGGT
TCGATGGAGATTTAGGTTGACAAACCAAGACTCGTAGCTTCTCTTGTTGCACTCTTTATT
GATGAGCCTCAATTTTCCGATTTCGGACC

Last Updated on 06.12.2018 16:11