Sequences from confirmation amplicon


Confirmation sequences for line 804C07 and hit At1g58340 are shown.
Line ID 804C07
Locus At1g58340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21654678
Primers used for PCR RU78 - 8474, show primer details
   
Sequence Name 63-K027349-022-804-C07C-8409
GenBank Accession LN510897 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K027349-022-804-C07C-8409
GCCATTTACAATGGTGCCTAACCAGCACAGCAAACACCATCGCCATTAGGCCTAAGGCGA
TCGCGCAGAAGAGTGAGATGATCATGGATACCCGAGCTTTCGCCGGACGTTTAGCCCCAA
GCTCGTTGCTAATTCTTGTAGAGACACCAAGGCTGAGAGAGGATGGGAAGACGTAGACCA
AAGCCGTCGTTTGGATCAAGATTCCCATGGAAGCCACGGTGGCTCTTGGGTTAGCCAAAA
GTCCACACAAAATGATCATAAACTCGTACCACCACCACTCCAAACAAACAGATACACAAG
TGGGTATGGCAAGTGAGAGCAAAGCCGACCAACCTTTAAGAGAGTCAATAGTAATTGGGA
CCCATGTATCACTATGCACGCTAGTGAAATACACAAAAGAGGATAAGAGGACTACAAGGT
TGAGATTTGTCAAAACCATAGCTATGGCGACT
   
Sequence Name 64-K027349-022-804-C07C-RU78
GenBank Accession LN511111 [GenBank]
Sequencing primer gene specific primer, RU78: 5'-GGAGTCGCTATAGCTATGGTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K027349-022-804-C07C-RU78
CTCTTTTGGATTTCACTAGCGTGCATAGTGATACATNGGGTCCCAATTACTATTGACTCT
CTTAAAGGTTGGTCGGCTTTGCTCTCACTTGCCATACCCACTTGTGTATCTGTTTGTTTG
GAGTGGTGGTGGTACGAGTTTATGATCATTTTGTGTGGACTTTTGGCTAACCCAAGAGCC
ACCGTGGCTTCCATGGGAATCTTGATCCAAACGACGGCTTTGGTCTACGTCTTCCCATCC
TCTCTCAGCCTTGGTGTCTCTACAAGAATTAGCAACGAGCTTGGGGCTAAACGTCCGGCG
AAAGCTCGGGTATCCATGATCATCTCACTCTTCTGCGCGATCGCCTTAGGCCTAATGGCG
ATGGTGTTTGCTGTGCTGGTTAGGCACCATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11