Sequences from confirmation amplicon


Confirmation sequences for line 805C04 and hit At4g22430 are shown.
Line ID 805C04
Locus At4g22430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11828065
Primers used for PCR GA50 - 8474, show primer details
   
Sequence Name 38-K101384-0022-805-C04C-8409
GenBank Accession LN502915 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K101384-0022-805-C04C-8409
GCCTTTACATTGTAATCCCTGCCTGTCCGGTTCATATACTTTCTTTTGTGGTGTGGTGAC
GGGTGTTGACGAGGTGGGTGTTGTTTTGAGCTTCAAAGTGGTTAAGAGTGGTAATATGTT
TCTTAATAAAGGTGAAATGTATATGCCTTTATATGTGTATTCGTCTGAGACAGGCTTTTG
GATTCACAAAGAAGTTGTATGCCCTGTTCGCTTACCCAACTTCTATGACCCTATTAGTTT
GAATGGGACGCTTTATTTTTCGCAACGGGGGGATTCTTATAATCGTAGGCCAGGACTTAT
GGTTCTCGATTTCTATGGTAAACCCAAAGATTGTCACTTTATACCGCTCCCCGATCATGC
TTTGAATCGCAACAAAACATGTTTGGCTACATCCAGCGGTTTTGTCATGTATATCANAAC
ATTAGCTCAACCGGGAGGTAATCTTTTGAAGGGTTGGAGGTTGATTGATGATTCAGCTTG
GGCACTTATGTGGGGAGTCAGCATCCCATTTATTGGATGTTATGCAC
   
Sequence Name 38-K101394-0022-805-C04C-GA50
GenBank Accession LN502916 [GenBank]
Sequencing primer gene specific primer, GA50: 5'-ATGGGTGCATAACATCCAATAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K101394-0022-805-C04C-GA50
CAGCTGAATCATCAATCAACCTCCAACCTTCAAAGATTACCTCCCGGTTGAGCTAATGTT
TTGATATACATGACAAAACCGCTGGATGTAGCCAAACATGTTTTGTTGCGATTCAAAGCA
TGATCGGGGAGCGGTATAAAGTGACAATCTTTGGGTTTACCATAGAAATCGAGAACCATA
AGTCCTGGCCTACGATTATAAGAATCCCCCCGTTGCGAAAAATAAAGCGTCCCATTCAAA
CTAATAGGGTCATAGAAGTTGGGTAAGCGAACAGGGCATACAACTTCTTTGTGAATCCAA
AAGCCTGTCTCAGACGAATACACATATAAAGGCATATACATTTCACCTTTATTAAGAAAC
ATATTACCACTCTTAACCACTTTGAAGCTCAAAACAACACCCACCTCGTCAACACCCGTC
ACCACACCACANAAGAAAGTATATGAACCTGGACAGGCAGGGATTTACAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCCATTTTTTTTTCATTTCAAAATGTAGATATCCGCAG

Last Updated on 06.12.2018 16:11