Sequences from confirmation amplicon


Confirmation sequences for line 807E01 and hit At3g43430 are shown.
Line ID 807E01
Locus At3g43430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:15354842
Primers used for PCR DA26 - 8474, show primer details
   
Sequence Name 88-K107706-0022-807-E01C-DA26
GenBank Accession LN514871 [GenBank]
Sequencing primer gene specific primer, DA26: 5'-CTCCCTCTTCTTCTCCTCTCTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K107706-0022-807-E01C-DA26
TCTTCTCGAAGACCTAGTCTCATCATCAGCCACGTTCTCTACAAAACGGCACTTATCATC
ACCGTTTTACGATGGATCTTTGCATGGATCCTCCGTTACCGCTCAAGATCCTCCTCATCA
TCATCATCTCAATCCTCCTCTTCTCCTTCTATTTCATCACAAACCATCAAAGAAAGCCTA
GCCGTGTCCGCTTTCCGCGACGCGGTGGAGAGATCTCCCGCGGCGATAAACGACATGTGT
GCCGTGTGTCTAGGAGATCTTGAAGACGAGGACGAAATCAGAGAGCTTAGGAACTGTACT
CACGTGTTTCATCGTGATTGTATTGACCGATGGCTAGATTATGAATGTTGCGGCGGTGAT
GATGATAACCACCGTACTTGTCCCCTATGTCGAACGCCGTTGCTTCCATCTACATGTATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTA
   
Sequence Name 72-K107705-0022-807-E01C-8409
GenBank Accession LN512736 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K107705-0022-807-E01C-8409
TTACATTGAATAATGTAGATGGAAGCAACGGCGTTCGACATACGGGTACAAGTACGGTGG
TTATCATCATCACCGCCGCAACATTCATAATCTAGCCATCGGTCAATACAATCACGATGA
AACACGTGAGTACAGTTCCTAAGCTCTCTGATTTCGTCCTCGTCTTCAAGATCTCCTAGA
CACACGGCACACATGTCGTTTATCGCCGCGGGAGATCTCTCCACCGCGTCGCGGAAAGCG
GACACGGCTAGGCTTTCTTTGATGGTTTGTGATGAAATAGAAGGAGAAGAGGAGGATTGA
GATGATGATGATGAGGAGGATCTTGAGCGGTAACGGAGGATCCATGCAAAGATCCATCGT
AAAACGGTGATGATAAGTGCCGTTTTGTAGAGAACGTGGCTGATGATGAGACTAGTGTCT
TCGATGAAGAATGTCATTGCGAAAACTTGGAGTAGATAAAGAGAGGAGAAGAAGAGGGA

Last Updated on 06.12.2018 16:11