Sequences from confirmation amplicon


Confirmation sequences for line 808B07 and hit At2g22420 are shown.
Line ID 808B07
Locus At2g22420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9513545
Primers used for PCR 57hw - 8474, show primer details
   
Sequence Name 46-K112776-0022-808-B07C-57hw
GenBank Accession LN505914 [GenBank]
Sequencing primer gene specific primer, 57hw: 5'-CTTCTTACGGTAACTCGGTTCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K112776-0022-808-B07C-57hw
CGGATTGGTATAAGTCTGAACATGATTGAAAACACCGACCTTGACCTATTGAGTGTGACC
CTGATAAAGCAACCATGTCTTTTACAGAAAGATTGAATCTTTCGAAGAGATCAATCAAGA
AAGTTGCGTTGGCTCTTGGACTTGGCATTATATCGTCGGAATCTTGCTGACTCGCTGTTA
AACTGTCTTTCCTCCCTAGCTTCACTTCCCAATCTGGTCCTCCTGTCTGGTTTTTTCAAA
GACCAAAATTACAAAACCGAGATTTCACTAAACCGGGGAAATTGAATTTTGACTTACAAG
AGCAACGGCGTCACGAGCGGCCATGATAACGATATCGGCACAAGAAACAGTAGCTGGACA
AGCTTTCTCTAGAGCTTCTTTAATGTCGTCAACGACTTCGAAAGATCTTAGTGAATCGAT
GTTGGAAAGTGATAGTTTTTCACCAAGCATGTTTGGTGTGTCGTCAAGCAACAGAGATGC
GTCACATCCCTGAATTTGATGATTCAAAGAAAAATTCAGAGAGGAAAACAGAGTAAAGAT
CAAGAACAGAGAGAAAGATTTATAGTGTTTACCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 23-K112774-0022-808-B07C-8409
GenBank Accession LN496626 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K112774-0022-808-B07C-8409
TTGGTAAACACTATAAATCTTTCTCTCTGTTCTTGATCTTTACTCTGTTTTCCTCTCTGA
ATTTTTCTTTGAATCATCAAATTCAGGGATGTGACGCATCTCTGTTGCTTGACGACACAC
CAAACATGCTTGGTGAAAAACTATCACTTTCCAACATCGATTCACTAAGATCTTTCGAAG
TCGTTGACGACATTAAAGAAGCTCTAGAGAAAGCTTGTCCAGCTACTGTTTCTTGTGCCG
ATATCGTTATCATGGCCGCTCGTGACGCCGTTGCTCTTGTAAGTCAAAATTCAATTTCCC
CGGTTTAGTGAAATCTCGGTTTTGTAATTTTGGTCTTTGAAAAAACCAGACAGGAGGACC
AGATTGGGAAGTGAAGCTAGGGAGGAAAGACAGTTTAACAGCGAGTCAGCAAGATTCCGA
CGATATAATGCCAAGTCCAAGAGCCAACGCAACTTTCTTGATTGATCTCTTCGAAAGATT
CAATCTTTCTGTAAAAGACATGGTTGCTTTATCAGGGTCACACTCAATAGGTCAAGGTCG
GTGTTTTTCAATCATGTTCAGACTTTATAACCAATCCGGTTCTGGTAAACCGGATCCAGC
TCTTGAACCG

Last Updated on 06.12.2018 16:11