Sequences from confirmation amplicon


Confirmation sequences for line 810E08 and hit At3g20350 are shown.
Line ID 810E08
Locus At3g20350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7097171
Primers used for PCR 34qn - 8474, show primer details
   
Sequence Name 16-K115694-0022-810-E08C-34qn
GenBank Accession LN493566 [GenBank]
Sequencing primer gene specific primer, 34qn: 5'-GACTCAGACAAAGGAATAGCCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K115694-0022-810-E08C-34qn
TTCAGATTACAACTATAGTGAAGCTAAGGCAGTATTTTCATACTCCGACCAGACGGTTAA
ACCGACCGAGGTCAGATTTACTAGCTCAAGTAACAACTCTAAACTCATAGAACCTTCATC
TCAACCACAACATTTAGTTATGGTTCTTAATAAAGCACCTCAAGTAACATGTATAAGACT
TTTTGAAGCATATCCACATCTAATTGAATTGACATAATATAATCTTCACCTAGAAGTTTG
AGATTATCAACAAACCTTACACAAGAATCTACTATGCTTGTTTCTTGAATTGTTGCTTTG
AAAGCCAGAATGTTTGTCATCATGGTGGTGATAATAAAACAGAGGACCCAAGTTTCCAGC
AGGACCAGCAGTTTCCTGGAGAAAAACACCAAAGAAAATAAAGAGAAATTTATTTGACAT
ATGAACTCCTCTGAGATAGTAGCTAAAGCCAACTAATTCAGATCCATCACACAAGAAACA
GAGTGTACAGATTCCAGAGACATGAGCAAACAGTTATGAACTAGTGTGAAGATCCTGGAA
TCAACAGTCATTTCAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 07-K115693-0022-810-E08C-8409
GenBank Accession LN489413 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K115693-0022-810-E08C-8409
CGGATGAGCCTTTACATTGAATATATCCTGTTGAATGACTGTTGATTCCAGGATCTTCAC
ACTAGTTCATAACTGTTTGCTCATGTCTCTGGAATCTGTACACTCTGTTTCTTGTGTGAT
GGATCTGAATTAGTTGGCTTTAGCTACTATCTCAGAGGAGTTCATATGTCAAATAAATTT
CTCTTTATTTTCTTTGGTGTTTTTCTCCAGGAAACTGCTGGTCCTGCTGGAAACTTGGGT
CCTCTGTTTTATTATCACCACCATGATGACAAACATTCTGGCTTTCAAAGCAACAATTCA
AGAAACAAGCATAGTAGATTCTTGTGTAAGGTTTGTTGATAATCTCAAACTTCTAGGTGA
AGATTATATTATGTCAATTCAATTAGATGTGGATATGCTTCAAAAAGTCTTATACATGTT
ACTTGAGGTGCTTTATTAAGAACCATAACTAAATGTTGTGGTTGAGATGAAGGTTCTATG
AGTTTAGAGTTGTTACTTGAGCTAGTAAATCTGACCTCGGTCGGTTTAACCGTCTGGTCG
GAGTATGAAAATACTGCCTTAGCTTCACTATAGTTGTTAACTTGAACACTTTAAGACATT
GACACGCTGCACATTTCTTGGTTGGCTATTCCTTTGTCTGAGT

Last Updated on 06.12.2018 16:11