Sequences from confirmation amplicon


Confirmation sequences for line 811F03 and hit At5g63930 are shown.
Line ID 811F03
Locus At5g63930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25585361
Primers used for PCR KQ91 - 8474, show primer details
   
Sequence Name 39-K101715-0022-811-F03C-8409
GenBank Accession LN503300 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K101715-0022-811-F03C-8409
TTTACATTGAGTATATGTCCAAGACATTTCCGATGGTTGGCCATCTTGAGCAGAAGAAGC
GACTGTTCTCACTGGGCGTCTCATGAGGTAGACAATGAGAGCAATCAGCATGAGAGAGAC
CCCGCCAATCACAGCTGCAGTAATGGCTATGATTTTACTCGAACGCATTCCTCCGGGCTT
CCCGGTTGACTGAGAAGGCGCGAAAGGCTGTGTTTGGATACACTGGTTGAGCGGCGGGCC
GCAAAGCCCTTCATTACCGATGAAGCTAGACATGGATATGTTACGGAGAAGTGGAATAGG
ACCAGTTAAGCTGTTGTATGAGAAGTTGTAACCAAGCAAGCTGGAGAGATTAGCAAAAGA
GCTTGGTATTTCCCCAGACAAATTGTTGTTGTTGAGGAGCAGGAACTCAAGCATAACAAG
ATTACTGAGCTCAGGTGGTATTTCTCCGGTAAGCTTGTTATAACTGAGGTTCAATGCTAT
TTGCAAACCCGTCAGACTGCCCAACTCCCGTGGAATGCTACCGTTGAACAAGTTTCCACC
CATTTGTAGTTCGGTCAAGCGAGATAAGTTCCCTAGAGCCACAGGTATTGT
   
Sequence Name 39-K101721-0022-811-F03C-KQ91
GenBank Accession LN503301 [GenBank]
Sequencing primer gene specific primer, KQ91: 5'-AATCTTTCGGGTACAATACCTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K101721-0022-811-F03C-KQ91
CTGACGACTAAATGGGTGGAACTTGTTCACGGTAGCATTCCACGGCATTTGGGCAGTCTG
ACGGGTTTGCAAATAGCATTGAACCTCAGTTATAACAAGCTTACCGGAGAAATACCACCT
GAGCTCAGTAATCTTGTTATGCTTGAGTTCCTGCTCCTCAACAACAACAATTTGTCTGGG
GAAATACCAAGCTCTTTTGCTAATCTCTCCAGCTTGCTTGGTTACAACTTCTCATACAAC
AGCTTAACTGGTCCTATTCCACTTCTCCGTAACATATCCATGTCTAGCTTCATCGGTAAT
GAAGGGCTTTGCGGCCCGCCGCTCAACCAGTGTATCCAAACACAGCCTTTCGCGCCTTCT
CAGTCAACCGGGAAGCCCGGAGGAATGCGTTCGAGTAAAATCATAGCCATTACTGCAGCT
GTGATTGGCGGGGTCTCTCTCATGCTGATTGCTCTCATTGTCTACCTCATGAGACGCCCA
GTGAGAACAGTCGCTTCTTCTGCTCAAGATGGCCAACCATCGGAAATGTCTTTGGACATA
TACTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGA
TGTCCGCA

Last Updated on 06.12.2018 16:11