Sequences from confirmation amplicon


Confirmation sequences for line 812G08 and hit At5g09840 are shown.
Line ID 812G08
Locus At5g09840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3061689
Primers used for PCR 51kh - 8474, show primer details
   
Sequence Name 76-K113756-0022-812-G08C-51kh
GenBank Accession LN513436 [GenBank]
Sequencing primer gene specific primer, 51kh: 5'-AACCCCATCTTCTTCTTTACCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K113756-0022-812-G08C-51kh
ACCGTCTGCTTCAACGGTTTGAGCTCAATCTTCTCGGATTCTTCAAATCACAGTGGCAGA
AACAAAATGGTGAGAAAAGCGGCAAGAACGTTGCTCATGCTGGAGTTTCTGTTGGTTCTG
TGGACCGGAAAGAGCTGGAAAGATACAAAAGTAATGCAATAGCGGATTGTCAGAAGATGA
TAAAGAAGATAACAGAGGAACACCCTGAAGGGTATAGCCTGATTCGTTTCAGGAAAGACT
TCTTAGAGGAGTATGGGTACCATTTAGCGGTTGACAAGCTCGGTTACGACAACCTGCAGT
CTCTGATTCGAGTCATGCATGGAGTGAGAATAGCATCTGGATACATCTTTCCTTCAACTC
CTTCACCAAATGCCAAGTCAAAAGAAGATGATTCGGACTTGGCATTCGCAGAACTTGGTC
CAGTTTCTGATACAAAAAAGCAATTACAAGAAAAATGAACTTACCGTTCATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 38-K113755-0022-812-G08C-8409
GenBank Accession LN503053 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K113755-0022-812-G08C-8409
GCCTTTACATGAACGGTAAGTTCATTTTTCTTGTAATTGCTTTTTTGTATCAGTAACTGG
ACCAAGTTCTGCGAATGCCAAGTCCGAATCATCTTCTTTTGACTTGGCATTTGGTGAAGG
AGTTGAAGGAAAGATGTATCCAGATGCTATTCTCACTCCATGCATGACTCGAATCAGAGA
CTGCAGGTTGTCGTAACCGAGCTTGTCAACCGCTAAATGGTACCCATACTCCTCTAAGAA
GTCTTTCCTGAAACGAATCAGGCTATACCCTTCAGGGTGTTCCTCTGTTATCTTCTTTAT
CATCTTCTGACAATCCGCTATTGCATTACTTTTGTATCTTTCCAGCTCTTTCCGGTCCAC
AGAACCAACAGAAACTCCAGCATGAGCAACGTTCTTGCCGCTTTTCTCACCATTTTGTTT
CTGCCACTGTGATTTTGAAGAATCCGAGAAGATTGAGCTCAAACCGTTTGAAGCATGACG
GTGGCCAGGGCTAGATCCTTTGATGATTCTGAATGGTAAAGAAGAGAATGGGGT

Last Updated on 06.12.2018 16:11