Sequences from confirmation amplicon


Confirmation sequences for line 813E08 and hit At1g26380 are shown.
Line ID 813E08
Locus At1g26380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9127664
Primers used for PCR BC25 - 8474, show primer details
   
Sequence Name 43-K103981-0022-813-E08C-BC25
GenBank Accession LN504814 [GenBank]
Sequencing primer gene specific primer, BC25: 5'-ATCTCTTCAAATTCCCCAAGAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K103981-0022-813-E08C-BC25
GTCTGTCTCCCGCTTGGATTGCTTCCATATCAATGTCTCTGTAATTAAAGAAGGCCTCTC
TCGGGTTACTTGACACGTACGGTTCTGCAACCTCGTAGAGCTCCTTCATAATACTCAGGT
TACTCTCTGTGGCGTTTGGGTCCAACCATGTGGTATAGTATTGAACCTTGAACAAGTTTC
CTTTCCGGTGAGGAAACGCTGTGGCGTTTGACGGAATCCGATCCATCATTCCACCGTAAG
GGTTGAAGTGCAGCCACACAATATTGTTGAATTTCAACATTGTCTTCCAAATCTTCTCCA
GTCCTTCTTTGGGGATTGGCTTTTTGACGTAATCCGATTTACTCTTAAAGAATACTGGAT
CCGTAGGTCTACCTAGAAGGACGGTCTTCGGAGTACCCGCTGGTAACATAGCCCAAAACA
ACGTCGTGTTAAGCCAGCTCATTTCTTGACAATCTTCACGTCTTAGCCCTAATTCAGGAA
AGCTCTGGTTCATTATCTCCATCAGCTCATCGGTTCGACCCAAGAACTGAGCCCAGAATA
CAATCGCGATGGTTCTCTCGCCGTGTTTTGTTCCATTTACTACCTGAGGCATTGCTCTCA
AGAAAATCTACGTGAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAG
   
Sequence Name 59-K103982-0022-813-E08C-8409
GenBank Accession LN509984 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K103982-0022-813-E08C-8409
GGACTGAGCCATTTCAATTGAAATTTCACGTAGATTTTCTTGAGAGCACCGCTTCAGGTA
GTAAATGGAACAAAACACGGCGAGAGAACCATCGCGATTGTATTCTGGGCTCAGTTCTTG
GGTCGAACCGATGAGCTGATGGAGATAATGAACCAGAGCTTTCCTGAATTAGGGCTAAGA
CGTGAAGATTGTCAAGAAATGAGCTGGCTTAACACGACGTTGTTTTGGGCTATGTTACCA
GCGGGTACTCCGAAGACCGTCCTTCTAGGTAGACCTACGGATCCAGTATTCTTTAAGAGT
AAATCGGATTACGTCAAAAAGCCAATCCCCAAAGAAGGACTGGAGAAGATTTGGAAGACA
ATGTTGAAATTCAACAATATTGTGTGGCTGCACTTCAACCCTTACGGTGGAATGATGGAT
CGGATTCCGTCAAACGCCACAGCGTTTCCTCACCGGAAAGGAAACTTGTTCAAGGTTCAA
TACTATACCACATGGTTGGACCCAAACGCCACAGAGAGTAACCTGAGTATTATGAAGGAG
CTCTACGAGGTTGCAGAACCGTACGTGTCAAGTAACCCGAGAGAGGCCTTCTTTATTACA
GAGACATTGATATTGGAAGCAATCCCAGCGGTGAGACAGACGTGGATGAGGCTAAGATCT
ATGGATACAAGTATTTC

Last Updated on 06.12.2018 16:11