Sequences from confirmation amplicon


Confirmation sequences for line 813G04 and hit At2g18915 are shown.
Line ID 813G04
Locus At2g18915 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8197033
Primers used for PCR BD71 - 8474, show primer details
   
Sequence Name 09-K028744-022-813-G04C-8409
GenBank Accession LN490040 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K028744-022-813-G04C-8409
ATTGAATATATCCTGGGCGACCACAAACCCACACGGCGCTCGCGAAACGGAAGACTACCT
ACCGGAAACGGAATCGCTCCGTTATTCTCCACCGAACCATCCATCCTCCGCCACTTCATC
TCCACCGCTGAGATCGGAATCACTATCCCACTCCATTTGATTTTGCATACACACCCACAA
AATAAAGCTTAAGCCCACTAATCTTCTTCCTCAGCAACAGGAGAAGAGACCCAGAAGAAC
AAAGTTTGATTTTGAATCGAGGAATCTGATATCTGTAGGTAATCGAAGTCTCCACAGGAA
AAAAGTTCCAAACTTTAGACAAACCCAGAAATCGTCTTCTTCAGGAACAGGAGATAGAAG
AACAGATTTTGAAATGGAATCGAGCGAATAAAAGAGGAATCTGATATTTGTAGGTAATGG
AAGTCTCCACCGCCCACTTCTAAGAGGAGGAGAAGAAGAAGAAGAGAGATGATTCGGTCG
GTCGATGACTCGGCTCTTCTCAGTGCCTCTCCTTTTCCATCTNTAACCA
   
Sequence Name 09-K028744-022-813-G04C-BD71
GenBank Accession LN490041 [GenBank]
Sequencing primer gene specific primer, BD71: 5'-TCTGGTTAAAGATGGAAAAGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K028744-022-813-G04C-BD71
GACGAATATCTCTCTTCTTCTTCTTCTCCTCCTCTTAGAAGTGGGCGGTGGAGACTTCCA
TTACCTACAAATATCAGATTCCTCTTTTCTTCGCTCGATTCCATTTCAAAATCTGTTCTT
CTATCTCCTGTTCCTGAAGAAGACGATTTCTGGGTTTGTCTAAAGTTTGGAACTTTTTTC
CTGTGGAGACTTCGATTACCTACAGATATCAGATTCCTCGATTCAAAATCAAACTTTGTT
CTTCTGGGTCTCTTCTCCTGTTGCTGAGGAAGAAGATTAGTGGGCTTAAGCTTTATTTTG
TGGGTGTGTATGCAAAATCAAATGGAGTGGGATAGTGATTCCGATCTCAGCGGTGGAGAT
GAAGTGGCGGAGGATGGATGGTTCGGTGGAGATAACGGAGCGATTCCGTTTCCGGTAGGT
AGTCTTCCTGGCACGGCGCCGTGTGGGTTTGTGGTCGCCAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
Primers used for PCR BD70 - 8474, show primer details
   
Sequence Name 17-K028745-022-813-G04C-8409
GenBank Accession LN493719 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K028745-022-813-G04C-8409
CCGAAGAAGTTATTGGTCGAAATTCGGTGAGTTTGCTCTTCTCGACTTTTACCCTAAATT
TGTGGCGTCGATTTTGTTTTACTGTAGCGAATTTTGTCTCATTTGTGGTATTGGTACGAT
GTTACTAGAAAGCTTTGTTGAAAGCTGTTTTGACAGAAAGAAATTTATATTTCGATTCAT
TATTATGGTTCTTGTTGATTATGTTAGGTATTATTAGCTAGAACCATTTTGGAATCATGG
GTTGGTTATTGAATTAGAAGTTTGTTGTGGAGGAACTAAGCTATAATGTGAGGTTCAGTT
TGTAATGTTTCCTATGTCTGTGGATTAGTGTTTAGGAACTCGAACTTTGATAGTTTTTCA
CATGTCTTGATTGGTTTATGAAAGCCTTGGGATTATTGATTTTTGTAATATTGAATTGTA
ATGCGTTTTTGTCTAAGTATATATGTTTCTGTTGAATATGTTTCTCTTGGGTTTTAGCGG
CTTTTGATCTGGTATTCCATGTTAATTATGCATATAGTTTAGTCTTTGTTCAATACTTGT
GTGTGAGGAATTGTTCG
   
Sequence Name 17-K028745-022-813-G04C-BD70
GenBank Accession LN493720 [GenBank]
Sequencing primer gene specific primer, BD70: 5'-CAGATACAGTACTTTGGCGAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K028745-022-813-G04C-BD70
AAACTATATGCATAATTAACATGGAATACCAGATCAAAAGCCGCTAAAACCCAAGAGAAA
CATATTCAACAGAAACATATATACTTAGACAAAAACGCATTACAATTCAATATTACAAAA
ATCAATAATCCCAAGGCTTTCATAAACCAATCAAGACATGTGAAAAACTATCAAAGTTCG
AGTTCCTAAACACTAATCCACAGACATAGGAAACATTACAAACTGAACCTCACATTATAG
CTTAGTTCCTCCACAACAAACTTCTAATTCAATAACCAACCCATGATTCCAAAATGGTTC
TAGCTAATAATACCTAACATAATCAACAAGAACCATAATAATGAATCGAAATATAAATTT
CTTTCTGTCAAAACAGCTTTCAACAAAGCTTTCTAGTAACATCGTACCAATACCACAAAT
GAGACAAAATTCGCTACAGTAAAACAAAATCAACACCACAAATTTAGGGTAAAAGTCAAG
AAGAGCAAACTCACCAATTTCGACCAATAACTTCTTCAGCTCGATAACCAGTAACAATCT
CAAAAATGATATAAATGGCTATATTCAATTGAATATATAAATTGACCAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11