Genotyping and primer details


Genotyping and primer details for 813G04 - At2g18915
Line ID 813G04
Locus At2g18915 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8197033 of line 813G04 Chr2:8197033
   
Insertion allele amplicon 8474 - BD70
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BD70: 5'-CAGATACAGTACTTTGGCGAACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8196463
   
Insertion allele amplicon 8474 - BD71
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BD71: 5'-TCTGGTTAAAGATGGAAAAGGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8197618
   
wt amplicon (over InsPos) 02jw - BD70
wt amplicon start Chr2:8196463
wt amplicon end Chr2:8197518
wt amplicon size 1056 bp
Locus specific primer BD70: 5'-CAGATACAGTACTTTGGCGAACAA-3'
2nd locus specific primer 02jw: 5'-GGTGGAGACTTCCATTACCTACAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11