Sequences from confirmation amplicon


Confirmation sequences for line 815D06 and hit At1g11650 are shown.
Line ID 815D06
Locus At1g11650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3917122
Primers used for PCR DA33 - 8474, show primer details
   
Sequence Name 38-K107369-0022-815-D06C-DA33
GenBank Accession LN503002 [GenBank]
Sequencing primer gene specific primer, DA33: 5'-GTGAGAGAAGCTGCGATAGTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K107369-0022-815-D06C-DA33
TGTCAATGTAAAGATTATCAATTACCTGTTTGTTCGAAGGACTTCGGCCCCATGAGAGCC
TGACGGTTGTTCCGCCTAATTGCACTCCATTCAGCATTCTAAGAGCTTCCTCTGCACAGC
TCCTGAAAAGTGAGGGTTGGTCACAAAACACATGATAAAAAAAACTATTAGAATTCTATT
CCAAAGACTATTTTAGGGTTAAATGAAAGACAGAGCTTCCAGTAGTAGCAGCAGACTTAC
TTCTCGGAAAACTGAACGAATCCACAGCGCTTTCCAGCGGGTATTTTCACATGCACAATC
TCACCATATTGGCTAAAGACATTCTTCAGATGATCATCCGTGACAGATGCATCTAATCCA
CCAACAAAAACCTGTACAGTAAATTAAAATGCGTCAGATATAACCGCTTTGGAAGATAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 54-K107370-0022-815-D06C-8409
GenBank Accession LN508498 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K107370-0022-815-D06C-8409
CGGAATGAGCCATTTTCTTCCAAAGCGGTTATATCTGACGCATTTTAATTTACTGTACAG
GTTTTTGTTGGTGGATTAGATGCATCTGTCACGGATGATCATCTGAAGAATGTCTTTAGC
CAATATGGTGAGATTGTGCATGTGAAAATACCCGCTGGAAAGCGCTGTGGATTCGTTCAG
TTTTCCGAGAAGTAAGTCTGCTGCTACTACTGGAAGCTCTGTCTTTCATTTAACCCTAAA
ATAGTCTTTGGAATAGAATTCTAATAGTTTTTTTTATCATGTGTTTTGTGACCAACCCTC
ACTTTTCAGGAGCTGTGCAGAGGAAGCTCTTAGAATGCTGAATGGAGTGCAATTAGGCGG
AACAACCGTCAGGCTCTCATGGGGCCGAAGTCCTTCGAACAAACAGGTAATTGATAATCT
TTACATTGACATTAGATGATAGAAAGAAACAGCTCACAGTGTATCGCCTTTTGTCTAAGA
ACTATCGCACTATCTCTCA

Last Updated on 06.12.2018 16:11