Sequences from confirmation amplicon


Confirmation sequences for line 816A09 and hit At5g60710 are shown.
Line ID 816A09
Locus At5g60710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24412350
Primers used for PCR 16do - 8474, show primer details
   
Sequence Name 45-K109938-0022-816-A09C-16do
GenBank Accession LN505558 [GenBank]
Sequencing primer gene specific primer, 16do: 5'-ATCTCTTTAGGAAGAAGGGCTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K109938-0022-816-A09C-16do
GGCTGTCTGGTATATGTATCTTGGCCATCGGATAAGAGTACGATGCTAGATACCGGGTTC
TTGAATCTACGATCGATCAAAACTCTTGCGCCCTTCTTCAATCCCTCTGCAATGTTAGTC
CCTCCGTTTGAGACCAATGAATTAACCGCCTGAAGCGCCTCTTGTTTACCAGTCTCGGTC
ATGAGACGAAGAGGGAAGTTACGACGTGCTGTGGAGGAGAAGGATATAACTGAGAGACGG
TCAAATGGACCAAGATTCTGAATCACAAAACCCATTGCTCGCTTTAGCAACGCCAGTTTT
GTTCCAGCCATGCTTCCACTCACATCAAGAACTGTGACTAGATCGACAGGAGCCCGAGAA
GACGAAGATGGATTTGAAGAAATATCGACAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCCAAAATGT
   
Sequence Name 37-K109937-0022-816-A09C-8409
GenBank Accession LN502647 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K109937-0022-816-A09C-8409
CATTTCATTGAATTATCCTGTCGATATTTCTTCAAATCCATCTTCGTCTTCTCGGGCTCC
TGTCGATCTAGTCACAGTTCTTGATGTGAGTGGAAGCATGGCTGGAACAAAACTGGCGTT
GCTAAAGCGAGCAATGGGTTTTGTGATTCAGAATCTTGGTCCATTTGACCGTCTCTCAGT
TATATCCTTCTCCTCCACAGCACGTCGTAACTTCCCTCTTCGTCTCATGACCGAGACTGG
TAAACAAGAGGCGCTTCAGGCGGTTAATTCATTGGTCTCAAACGGAGGGACTAACATTGC
AGAGGGATTGAAGAAGGGCGCAAGAGTTTTGATCGATCGTAGATTCAAGAACCCGGTATC
TAGCATCGTACTCTTATCCGATGGCCAAGATACATATACCATGACTAGCCCCAACGGTTC
CAGAGGGACGGATTACAAAGCCCTTCTTCCAA

Last Updated on 06.12.2018 16:11