Sequences from confirmation amplicon


Confirmation sequences for line 817C07 and hit At1g52400 are shown.
Line ID 817C07
Locus At1g52400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19517302
Primers used for PCR NB30 - 8474, show primer details
   
Sequence Name 60-K101327-0022-817-C07C-8409
GenBank Accession LN510207 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K101327-0022-817-C07C-8409
CGGACATGAGCCATTTACATTGAATATTCCTTGATTAATTTAGTGTATTTTTTTCCTTGA
ATTAGCCGTTTAATGGTAAACTGGATGTGTATTCAAAAGGTTTGAGATACCTTTTGAAGT
ATATTAAGGATAACTATGGCGACCCAGAAGTTATCATTGCCGAGAATGGTTAGAGAAAAT
ACTCAAAGCTCATTTCCTAAATTTCAATATTATTTTATTTTTGATGAAAAACGAAGTTTG
AATTTTTCTTTTGGTTTTCAAGGATACGGAGAAGACCTTGGAGAGAAGCACAATGACGTA
AACTTTGGGACACAAGATCACAACAGAAAATATTATATCCAAAGGCATCTCTTGAGTATG
CACGACGCCNTTTGGTAAGTAAAACTTCAAATTAAACAATCAAAACATAATTGAATCCCG
AAATAATTAATCTGATGATATTTTAACTGGTTATTAGCAAGGACAAAGTGAACGTTACGG
GATACTTTGTGTGGT
   
Sequence Name 60-K101333-0022-817-C07C-NB30
GenBank Accession LN510208 [GenBank]
Sequencing primer gene specific primer, NB30: 5'-ATCAAAGACCACACAAAGTATCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K101333-0022-817-C07C-NB30
TATACCAGTTAAATATCATCAGATTAATTATTTCGGGATTCAATTATGTCTTTAATGTTT
AATTTGAAGTTTTACTTACCAAATGGCGTCCTGCATACTCAAGAGATGCCTTTGGATATA
ATATTTTCTGTTGTGATCTTGTGTCCCAAAGTTTACGTCATTGTGCTTCTCTCCAAGGTC
TTCTCCGTATCCTTGAAAACCAAAAGAAAAATTCAAACTTCGTTTTTCATCAAAAATAAA
ATAATATTGAAATTTAGGAAATGAGCTTTGAGTATTTTCTCTAACCATTCTCGGCAATGA
TAACTTCTGGGTCGCCATAGTTATCCTTAATATACTTCAAAAGGTATCTCAAACCTTTTG
AATACACATCCAGTTTACCATTAAACGGCTAATTCAAGGAAAAAAATACACTAAATTAAT
CAAGGAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCCAAA
ATGTAGATTT

Last Updated on 06.12.2018 16:11