Sequences from confirmation amplicon


Confirmation sequences for line 818H10 and hit At5g37020 are shown.
Line ID 818H10
Locus At5g37020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14632015
Primers used for PCR 01sj - 8474, show primer details
   
Sequence Name 54-K116491-0022-818-H10C-01sj
GenBank Accession LN508596 [GenBank]
Sequencing primer gene specific primer, 01sj: 5'-AGCTGGAGATTCTGTCATTTTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K116491-0022-818-H10C-01sj
GATTATATTTGTACACTATTACTTGTTTCCAGGAATGAAAAGAATCAACTCTTTTTGGGA
ATTCGTCATGCCACTCGGCCGCAGACTATTGTACCATCATCTGTTTTATCTAGTGATAGC
ATGCATATTGGACTCCTTGCTGCTGCTGCACATGCTTCTGCAACTAATAGCTGTTTCACT
GTTTTCTTTCATCCAAGGTTGAAAAATCTCTCCCACCTGCATCTTTTTCATCTCTCGGTA
TATCCGATAATATATCCTACTAATATTCTCTTCATGTTCAGGGCTAGCCAATCTGAGTTT
GTGATACAACTTTCCAAGTACATTAAAGCCGTTTTTCACACGCGTATTTCAGTTGGGATG
CGCTTTCGCATGCTCTTCGAGACAGAAGAGTCGAGTGTCCGCAGGTGATGATGCCACATA
TCTGGAAATGCAATCTTGAAACTGAGAAAACTATATTACTATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCAACA
   
Sequence Name 22-K116490-0022-818-H10C-8409
GenBank Accession LN496270 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K116490-0022-818-H10C-8409
TTGAATATAGTAATATAGTTTTCTCAGTTTCAAGATTGCATTTCCAGATATGTGGCATCA
TCACCTGCGGACACTCGACTCTTCTGTCTCGAAGAGCATGCGAAAGCGCATCCCAACTGA
AATACGCGTGTGAAAAACGGCTTTAATGTACTTGGAAAGTTGTATCACAAACTCAGATTG
GCTAGCCCTGAACATGAAGAGAATATTAGTAGGATATATTATCGGATATACCGAGAGATG
AAAAAGATGCAGGTGGGAGAGATTTTTCAACCTTGGATGAAAGAAAACAGTGAAACAGCT
ATTAGTTGCAGAAGCATGTGCAGCAGCAGCAAGGAGTCCAATATGCATGCTATCACTAGA
TAAAACAGATGATGGTACAATAGTCTGCGGCCGAGTGGCATGACGAATTCCCAAAAAGAG
TTGATTCTTTTCATTCCTGGAAACAAGTAATAGTGTTACAAATATAATCACAATCCACAA
ACTGAACGTACAACAAAAGAACTAAACCTGATGAAAATGACAGACCCTCCAGCTA

Last Updated on 06.12.2018 16:11