Genotyping and primer details


Genotyping and primer details for 818H10 - At5g37020
Line ID 818H10
Locus At5g37020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:14632015 of line 818H10 Chr5:14632015
   
Insertion allele amplicon 8474 - 01sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01sj: 5'-AGCTGGAGATTCTGTCATTTTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:14631494
   
wt amplicon (over InsPos) 52vy - 01sj
wt amplicon start Chr5:14631494
wt amplicon end Chr5:14632692
wt amplicon size 1199 bp
Locus specific primer 01sj: 5'-AGCTGGAGATTCTGTCATTTTCAT-3'
2nd locus specific primer 52vy: 5'-ATGTTAGACCACTTCCCAAATCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11