Sequences from confirmation amplicon


Confirmation sequences for line 819D07 and hit At5g61560 are shown.
Line ID 819D07
Locus At5g61560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24753899
Primers used for PCR 28xx - 8474, show primer details
   
Sequence Name 51-K119798-0022-819-D07C-28xx
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 28xx: 5'-GAATTGATGACTCGATGTCGAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K119798-0022-819-D07C-28xx
AACCGGTTTACTTTGTGTACTGCAGAGTAAGTTTTTACCTGAATGGCTATCTGAAGAGAA
CCGGCTTTCGCTTCTATCGTCTGCAATGCTCGTTTCGGTGTCCATATCGGATTTACGAAC
ATTGAGAAGCTTTCCTTTAGAGATAACATGAACTGAGCAAAATCTTGGTGTAGCATCTGC
AATTCTTGAAGACAAGTTGCTTCTCTTCAACTTCCTACAAAAGGAAAAAGATTAAACAAT
CTGATGGAAATCTAAAGCTAAGAGATTGTGTTTTAAATGTTACCATGAGAAGATGATTGA
AGAGGAAGCTCCAATGACTAGCTCACTAATTCCATGATCTTGAACTGCTTTAGAGATTGC
ATCAGCTATATCGTCTGATTCAAGCACCATTATATCCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATTA
   
Sequence Name 15-K119797-0022-819-D07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K119797-0022-819-D07C-8409
CATTGGATATAATGGTGCTTGAATCAGACGATATAGCTGATGCAATCTCTAAAGCAGTTC
AAGATCATGGAATTAGTGAGCTAGTCATTGGAGCTTCCTCTTCAATCATCTTCTCATGGT
AACATTTAAAACACAATCTCTTAGCTTTAGATTTCCATCAGATTGTTTAATCTTTTTCCT
TTTGTAGGAAGTTGAAGAGAAGCAACTTGTCTTCAAGAATTGCAGATGCTACACCAAGAT
TTTGCTCAGTTCATGTTATCTCTAAAGGAAAGCTTCTCAATGTTCGTAAATCCGATATGG
ACACCGAAACGAGCATTGCAGACGATAGAAGCGAAAGCCGGTTCTCTTCAGATAGCCATT
CAGGTAAAAACTTACTCTGCAGTACACAAAGTAAAACCGGTTCCACCAGTTTGATTTGTT
ACACATTTTAGGAACAGTAAGTTCGACATCGATCAATCAATT

Last Updated on 06.12.2018 16:11