Sequences from confirmation amplicon


Confirmation sequences for line 820G11 and hit At1g72610 are shown.
Line ID 820G11
Locus At1g72610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27339492
Primers used for PCR 39xr - 8474, show primer details
   
Sequence Name 23-K119356-0022-820-G11C-39xr
GenBank Accession LN496779 [GenBank]
Sequencing primer gene specific primer, 39xr: 5'-GAATAATTGCAGAGGACCGATCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K119356-0022-820-G11C-39xr
CTGGTCCTAAAGTTAGCTTATGTTTGGAGTTTTTTTCTAAATTGGAATGTTCACAAATGT
CAATGCTTAGTTTTAGCTAATGATATGAACGGTGTGACCAACTCAGGATCTTAATTGAAA
AAACTCACATACAATGTAACAATTTTGACAGGTTTTGAAGAATTACAAGGCACACAAAAC
AGAGAAGACAAGAATCAATTAAAGAGAAAAAAAGATGAAGAAACAGGTTTTGGTGTTTTA
GTCAAGGAGTAGTCCTAAGTCTTTTTTTAGCCAGTTCCTCCAAGAACACCCTTTAGCTTC
TTGACTGTAGTGGCGTCAAGGAAAGTAGTACCCACGACGAGTTCAGTGGGAAGACTGTTA
GCAAAGAGTGCGAAGTCGAGAATCTGCAGACCCGGATTAGCGCTGTTGAAAGTGACAACG
GCTGAAGCAGAGGATTTTCCCGCGTTGATCTGGAAATGAACTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 03-K119355-0022-820-G11C-8409
GenBank Accession LN487705 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K119355-0022-820-G11C-8409
TATTTCCGATCAACGCGGGAAATCCTCTGCTTCAGCCGTTGTCACTTTCAACAGCGCTAA
TCCGGGTCTGCAGATTCTCGACTTCGCACTCTTTGCTAACAGTCTTCCCACTGAACTCGT
CGTGGGTACTACTTTCCTTGACGCCACTACAGTCAAGAAGCTAAAGGGTGTTCTTGGAGG
AACTGGCTAAAAAAAGACTTAGGACTACTCCTTGACTAAAACACCAAAACCTGTTTCTTC
ATCTTTTTTTCTCTTTAATTGATTCTTGTCTTCTCTGTTTTGTGTGCCTTGTAATTCTTC
AAAACCTGTCAAAATTGTTACATTGTATGTGAGTTTTTTCAATTAAGATCCTGAGTTGGT
CACACCGTTCATATCATTAGCTAAAACTAAGCATTGACATTTGTGAACATTCCAATTTAG
AAAAAAACTCCAAACATAAGCTAACTTTTAGGACCAGACTCTCACATGGGTTTCCAGTGG
TCCCAACTCCATAAAAGTTAGATCGGTCCTCTGCCAATTAT

Last Updated on 06.12.2018 16:11