Sequences from confirmation amplicon


Confirmation sequences for line 820G11 and hit At5g65430 are shown.
Line ID 820G11
Locus At5g65430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26150370
Primers used for PCR 35db - 8474, show primer details
   
Sequence Name 44-K109343-0022-820-G11C-35db
GenBank Accession LN505210 [GenBank]
Sequencing primer gene specific primer, 35db: 5'-CTTGTCTTCATATGCCTTTTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K109343-0022-820-G11C-35db
GCCGAAACGCATATGCTTAGCCAGCAAGGTAATAGCTTTAACAGGCTTCAAAAAGCCCAT
CTAAAGCCTAATCAAAAGCCCGTATTCTAACATTTGACCTTGAACAGTGTCAACTAGTTG
GGGAAGCTGACGCACTAAATTATTATCGTCCATTCACTCGCTCTTATCAAAAAAACCGAA
ACCTAGCCACGCGCCTCGTACACTCAACGTGGGTTTCACGCTGTTAATCCTTATTTCAAC
TAACGACGTCGTTTATCAGAGTGTGTTAATCACGCGCGAGTGAAGAGAATTTCCTTTTAC
AGCTCATTATGACCTGTATTCACTTGTAATAAATATCCTTTATGTGGTTGGCATGCACAT
ACAAATGGACGAACGGATAAACCTTTTCACGCCCTTTTAAATTTCCGTTTATGGCGTTGG
TGCATGCCCGGACATTTTACAATTGAATAAATATCCAATTTTCACATGAAGCCATTTACA
ATTGAATATTCCCGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 36-K109342-0022-820-G11C-8409
GenBank Accession LN502256 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K109342-0022-820-G11C-8409
GCCATTTACATTGAATTATCCTGTCCGGGAATATTCAATTGTAAATGGCTTCATGTGAAA
ATTGGATATTTATTCAATTGTAAAATGTCCGGGCATGCACCAACGCCATAAACGGAAATT
TAAAAGGGCGTGAAAAGGTTTATCCGTTCGTCCATTTGTATGTGCATGCCAAACACCTAA
AGGATATTTATTACAAGTGAATACAGGTCATAATGAGCTGTAAAAGGAAATTCTCTTCAC
TCGCGCGTGATTAACACACTCTGATAAACGACGTCGTTAGTTGAAATAAGGATTAACAGC
GTGAAACCCACGTTGAGTGTACGAGGCGCGTGGCTAGGTTTCGGTTTTTTTGATAAGAGC
GAGTGAATGGACGATAATAATTTAGTGCGTCAGCTTCCCCAACTAGTTGACACTGTTCAA
GGTCAAATGTTAGAATACGGGCTTTTGATTAGGCTTTAGATGGGCTTTTTGAAGCCTGTT
AAAGCTATTACCTTGCTGGCTAAGCATATGCGTTTTCGGCGTGACCTGGACACAACTAAT
GTGGTCGGAGAAAAGGCATATGAAGACA

Last Updated on 06.12.2018 16:11