Sequences from confirmation amplicon


Confirmation sequences for line 821A05 and hit At5g45080 are shown.
Line ID 821A05
Locus At5g45080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18192591
Primers used for PCR GG16 - 8474, show primer details
   
Sequence Name 51-K100853-0022-821-A05C-8409
GenBank Accession LN507432 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K100853-0022-821-A05C-8409
CCATTTACAATTGAATCTTGATGACCATTGAGTTTATGAAGTCATTATCGGTCCTCTCAC
TGCCATACCATACAAGTAAGAGATATCAAAAACAAACTGTTTATTATGTATATATATCTT
TAGGCCAAAAGAACATAAAAATAACCTTTGTTCAGCCAAGGGCATGCCTATTGATCCCGG
GATAGACTCTAATGCTTCCTTCCATTTCTGAGTTATTGGTAGCATGTGTCGATTATTGCG
TTCCAGAGCCCTGAAATTATCGCCAAATTTTCCTTCAAGAAATTTTACAGCGGAAGGTTT
AACTTTGTAGAAGATGGGAATCACTATGAGACGGCCTTGATCCTTGCGTTCCTTGATCTT
GGCCAGCTCATTCAAGCACCTATGCGATCTTGTGAAATCCACGGAAAATATCACTAGCGC
GAGCTCAGACTCTTCAATCCTCGTCAATAGGTTAGCCATTTCTGAGCCCAAAAACTCGTT
CTCATCTATGAAGACGTTTATGTTCGCTTCTCTCAAGGCAGGGACAAGAAAGCTTATGAA
GTGTTTGCGGACATCTGCTCCCCGGAAGCATATGAACACCTGAGGCCCCGTTGGGGTGGG
TTTCACAACCGAGGAAGAAGAAGCCATGAGAGCTTTTTTTGAGAAATAAGAAGAGAGTTT
TTGTTGATAATTGTTTGAGCTTCTGTTTTGTCAAGATGCTTTGGA
   
Sequence Name 51-K100859-0022-821-A05C-GG16
GenBank Accession LN507433 [GenBank]
Sequencing primer gene specific primer, GG16: 5'-TTGGATTAGTTTATCGTCCAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K100859-0022-821-A05C-GG16
ACATTATCAACAAACTCTCTTCTTATTTCTCAAAAAAGCTCTCATGGCTTCTTCTTCCTC
GGTTGTGAAACCCACCCCAACGGGGCCTCAGGTGTTCATATGCTTCCGGGGAGCAGATGT
CCGCAAACACTTCATAAGCTTTCTTGTCCCTGCCTTGAGAGAAGCGAACATAAACGTCTT
CATAGATGAGAACGAGTTTTTGGGCTCAGAAATGGCTAACCTATTGACGAGGATTGAAGA
GTCTGAGCTCGCGCTAGTGATATTTTCCGTGGATTTCACAAGATCGCATAGGTGCTTGAA
TGAGCTGGCCAAGATCAAGGAACGCAAGGATCAAGGCCGTCTCATAGTGATTCCCATCTT
CTACAAAGTTAAACCTTCCGCTGTAAAATTTCTTGAAGGAAAATTTGGCGATAATTTCAG
GGCTCTGGAACGCAATAATCGACACATGCTACCAATAACTCAGAAATGGAAGGAAGCATT
AGAGTCTATCCCGGGATCAATAGGCATGCCCTTGGCTGAACAAAGGTTATTTTTATGTTC
TTTTGGCCTAAAGATATATATACATAATAAACAGTTTGTTTTTGATATCTCTTACTTGTA
TGGTATGGCAGTGAGAGGACCGATNATGACTTCATAAACTCAATGGTCATCAAGATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCNAAATGTAGATT

Last Updated on 06.12.2018 16:11