Sequences from confirmation amplicon


Confirmation sequences for line 821H10 and hit At2g37840 are shown.
Line ID 821H10
Locus At2g37840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15852170
Primers used for PCR JL35 - 8474, show primer details
   
Sequence Name 60-K032524-022-821-H10C-8409
GenBank Accession LN510167 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K032524-022-821-H10C-8409
ATTTATAATCATCCTAACATCATCCGTCTGATCGACATGATCAAGGTTTCTTTTTTTGAT
TTGAAGTTTCGATTTTTTTGGTCGGTTCTCATTTTTTAGCTGAGAATTTTGAATTGAGGT
TAGCTTTGTTACTATATATGTTTCTCATTTGCTCATCCCAATGCCAATTTTGTGTCATAA
TGCCATGATTTTGCCGGTGAATTTTGGATCATATACATGGAATTAAGCTTCAATTGTGGA
ACAAAATTTGGGTCTAGTTTGATGTTGTTCTGATTTGGGCAATGTGATGCAGTCTCCTGG
GAAAGTACATTTGGTGTTGGAGTACTGCAAAGGAGGTGACTTGTCTGTGTATGTCCAAAG
ACATGGGATTGTCCCTGAAGCTACTGCTAAGCATTTCATGCAGCATNTAGGTAA
   
Sequence Name 60-K032524-022-821-H10C-JL35
GenBank Accession LN510168 [GenBank]
Sequencing primer gene specific primer, JL35: 5'-TTTACCTAACTGCTGCATGAAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K032524-022-821-H10C-JL35
AGACAAGTCACCTCCTTTGCAGTACTCCAACACCAAATGTACTTTCCCAGGAGACTGCAT
CACATTGCCCAAATCAGAACAACATCAAACTAGACCCAAATTTTGTTCCACAATTGAAGC
TTAATTCCATGTATATGATCCAAAATTCACCGGCAAAATCATGGCATTATGACACAAAAT
TGGCATTGGGATGAGCAAATGAGAAACATATATAGTAACAAAGCTAACCTCAATTCAAAA
TTCTCAGCTAAAAAATGAGAACCGACCAAAAAAATCGAAACTTCAAATCAAAAAAAGAAA
CCTTGATCATGTCGATCAGACGGATGATGTTAGGATGATTATAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
Primers used for PCR FQ50 - 8474, show primer details
   
Sequence Name 72-K118446-0022-821-H10C-FQ50
GenBank Accession LN512802 [GenBank]
Sequencing primer gene specific primer, FQ50: 5'-CCCGATCTTATCATATAAGCCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K118446-0022-821-H10C-FQ50
ATATCATTTTATTTCCTAACCTCTCTCTATCTCTCTCTTCATTATCCGAATTAAGCCAAT
CGAATCCTTTTCAACCCGACGGGTCGTGAAATTCCCCAAAAGATTCCTCTTTCTCAGCTC
AATTTCCAAAAGCTCCGACCTTTACGATCCTCTAGATTCTCGGCGCCGGTTTCTCGGAGT
AAATAATGGCTCAGTTCACGGGTCGGGTCGTTGGAGACTACTTGGTGGGTCGTCAAATCG
GGTCGGGTTCGTTCTCTGTTGTTTGGGAAGCGAGGCACCGCGTTGACGGTACTGAGGTTG
CTATCAAAGAGATCGCCATGGACAGGCTTAACAAGAAGCTGCAGGAAAGTCTCATGTCTG
AGATTTTTATCTTGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCACGGTTTATTA
   
Sequence Name 32-K118445-0022-821-H10C-8409
GenBank Accession LN500764 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K118445-0022-821-H10C-8409
GCCTTTACATTGAATATATCCTCAAGATAAAAATCTCAGACATGAGACTTTCCTGCAGCT
TCTTGTTAAGCCTGTCCATGGCGATCTCTTTGATAGCAACCTCAGTACCGTCAACGCGGT
GCCTCGCTTCCCAAACAACAGAGAACGAACCCGACCCGATTTGACGACCCACCAAGTAGT
CTCCAACGACCCGACCCGTGAACTGAGCCATTATTTACTCCGAGAAACCGGCGCCGAGAA
TCTAGAGGATCGTAAAGGTCGGAGCTTTTGGAAATTGAGCTGAGAAAGAGGAATCTTTTG
GGGAATTTCACGACCCGTCGGGTTGAAAAGGATTCGATTGGCTTAATTCGGATAATGAAG
AGAGAGATAGAGAGAGGTTAGTGAAATTAAAATGATTATTCTTAAGAATCTTCGTATAAT
TTGGCTTATATGATAAGATCGGG

Last Updated on 06.12.2018 16:11